摘要
目的探讨非穿透性小梁手术(nonpenetrating trabecular surgery,NPTS)对葡萄膜巩膜房水流出量的影响,从房水动力学的角度揭示NPTS降眼压的机制。方法用示踪剂异硫氰酸荧光素牛血清白蛋白(fluoresceinisothiocyanate-bovine serumalbumin:FITC-BSA)于术后7d分别对手术后的兔眼模型组和正常兔眼组进行前房持续灌注30min,灌注毕处死家兔,摘除双侧眼球,并将组织分离为前巩膜、后巩膜、前葡萄膜、后葡萄膜、视网膜和残余液体等6种组织。测定每种组织的荧光强度,计算葡萄膜巩膜流出量(uveoscleral outflow,Fu)。结果实验组葡萄膜巩膜流出量明显高于正常组,两组前房水再现量均以前葡萄膜、前巩膜和残余液体为多,实验组术后葡萄膜巩膜通道各组织房水再现量与正常组相比差异有统计学意义(p<0.001)。结论非穿透性小梁手术能增加葡萄膜巩膜途径房水流出量,房水主要由前巩膜排出。
Objective To study the effect of nonpenetrating trabecular surgery （NPTS）on the uveoscleral outflow（Fu） and demonstrate the physiological mechanisms of it. Methods Postoperative 7 days, rabbit eyes were perfused for 30 minutes with fluorescein-isothiocyanate bovine serum albumin （FITC-BSA） as a tracer, and then the rabbits were killed and the eyes were enucleated. After removing the cornea and iris, the eyes were dissected into anterior and posterior urea, anterior and posterior sclera, retina, and fluids. Fluorescent intensity of each tissue was determined and then Fu of each eye was calculated. Results Fu in experimental group was more than that in normal group remarkably, lweek postoperatively, Fu of each part in experimental group remarkably increased, and there,is significantly statistical difference compared with normal one（p〈0.001）. Conclusions NPTS can increase Fu in rabbit eyes. The aqueous humor is drained out of eyes mainly through anterior sclera.
出处
《实用防盲技术》
2008年第3期6-9,共4页
Journal of Practical Preventing Blind
