99B×海岛棉杂种2代的mtDNA RAPD分析

mtDNA-RAPD Analysis on Hybrid Second Generation of 99B×island Cotton

[目的]为mtDNA-RAPD技术检测杂种后代的纯度的可行性提供参考,为引物组合法广泛应用于植物纯度检测、遗传多样性分析提供依据。[方法]应用6对随机单引物和引物组合对99B和海岛棉及其F2代的mt DNA进行RAPD扩增并进行多态性分析。[结果]应用单引物对F2代mtDNA进行RAPD检测多态性分析,从6个单引物RAPD-PCR扩增得到了3个差异株,结果表明该群体的纯度为95%,应用引物组合扩增多态性分析表明,从15对引物RAPD-PCR扩增检测到4个差异株,该群体的纯度为93%。表明引物组合扩增多态性分析具有准确性高、精确性好的优点,也表明在植物种质纯度检测中引物组合法的优势及利用价值。[结论]mtDNA的多态性检测具有很好应用价值和应用前景。

[Objective]The aim was to provide reference for the feasibility of detecting the purity of hybrid progenies by mtDNA-RAPD technology and provide basis for the wide application of primer combination method in the plant purity detection and genetic diversity analysis. [Method] mt DNA of 99B, island cotton and their hybrid progeny F2 was amplified by RAPD-PCR using 6 random single-primers and primer combination and then analyzed the polymerphism. [ Result] The polymorphism analysis on the mtDNA of hybrid progeny F2 through RAPD detection by using single-primer showed that 3 different strains were obtained from 6 single primers after RAPD-PCR amplification and the purity of the population was 95%. The polymorphism analysis using primer combination showed that 4 different strains were detected from 15 pairs of primers after RAPD-PCR amplification and the purity of the population was 93%. The above result showed that the polymorphism ＂analysis ＂after amplification using primer combination had high accuracy and good vemci .ty and it also showed that primer combination method had advantages and utilization value in the purity detection of plant germplasm. [Conclusion] The polymorphism detection had good utilization value and application prospect.