基于Nafion/天青A/纳米金层层自组装固定酶的过氧化氢生物传感器

Hydrogen Peroxide Biosensor Based on the Layer-by-layer Self-assembly Immobilized Enzyme of Nafion,Azure A,Nano-gold

[目的]明确基于Nafion、天青A、纳米金层层自组装固定酶制备过氧化氢生物传感器的可行性。[方法]先利用静电吸附和自组装技术将Nafion、天青A、纳米金修饰到金电极表面,然后利用纳米金吸附辣根过氧化物酶(HRP),制备过氧化氢生物传感器。采用循环伏安法和计时电流法考察该传感器的性能。[结果]在测试溶液pH值5.5、工作电位-0.3^-0.2 V、扫速100 mV/s、检测温度25℃条件下,该传感器具有良好的生物催化活性,还原峰电流与H2O2浓度在2.0×10-6~1.6×10-3mol/L范围内呈良好的线性关系,检出限为7.62×10-7mol/L。该传感器稳定性好,抗干扰力强,制备方法简单,成本低廉,对H2O2有快速灵敏的响应。[结论]该研究制备的过氧化氢生物传感器灵敏度较高,检测范围宽,检出限较低。

[Objective] The research aimed to confirm the feasibility of preparing hydrogen peroxide biosensor based on layer-by-layer self-assembly immobilized enzyme of Nation,azure A,nano-gold. [Method] Nation,azure A and nano-gold were decorated on the golden electrode surface by using electrostatic adsorption and self-assembly technology. Horseradish peroxidase was adsorbed by using nano-gold to prepare hydrogen peroxide biosensor. The performances of this sensor were observed by using cyclic voltammetry method and chronoamperometry. [Result] Under the conditions that pH value of test solution was 5.5, working potential was - 0.3 N - 0.2 V, sweep speed was 100 mV/s and the detection temperature was 25 ℃, this sensor had good biology catalytic activity. The reduction peak current showed a good linear correlation with H2O2 cohen, in the range of 2.0 × 10^-6 - 1.6 × 10^-3 mol/L and the detection limit was 7.62 × 10^-7 mol/L. This sensor had good stability,strong anti-distmbance capability,simple preparing method, chcap cost and rapid and sensitive response to H2O2. [Conclusion] The prepared hydrogen peroxide biosensor had higher sensitivity,broad detection range and lower detection limit.