镉胁迫下泥鳅肝脏的红外光谱研究

Infrared Spectroscopic Study of Loach Liver under Cadmium Stress

[目的]研究镉胁迫对泥鳅肝脏的影响。[方法]使用FTIR-NEXUSTM傅立叶变换红外光谱仪在1800～400cm^-1范围内对泥鳅肝脏组织样品进行红外光谱扫描，将采集的红外光谱进行去卷积和曲线拟合处理后，分析Cd^2＋胁迫下泥鳅肝脏组织中相应大分子化合物特征基团的变化趋势。[结果]在Cd^2＋胁迫作用下，蛋白质、核酸的结构及合成途径都发生了明显变化。[结论]FTIR技术在动物抗逆研究及环境监测方面具有广泛的应用价值。

[ Objective] In order to study the influence of cadmium stress on loach liver. [ Method] FTIR-NEXUSTM Fourier transtonn inti＇ared spectrometer （FTIR） was used to scan liver sample with wavelength from 1 800 - 400 cm^-1. The collected infrared spectrum was conducted deconvolution and curve fitting treatment to analyze changing trend of relevant macromolecular compounds in loach liver tissue under Cd^2＋ stress. [ Result]The structures of protein and nucleic acid as well as their synthesis pathways changed significantly. [Conclusion]FTIR technique possessed broad application value in studying animal resistance and environment monitoring,