高效液相色谱法测定保健食品和饮料中维生素C

Determination of Vitamin C in Health Foods and Drinks by High Performance Liquid Chromatography

目的建立保健食品及饮料中维生素C的高效液相色谱检测方法。方法ODS C18 5μm反相色谱柱分离，流动相和试样提取液为0．1％草酸溶液。紫外检测器，流速1．0ml／min，检测波长262nm。结果线性范围为0．05—700．00μg／ml。加标回收率为84．2％-94．9％，RSD〈10％，检出限为0．05 μg／ml。结论本方法操作简便、快速、数据可靠，适合于饮料及保健食品中的维生素C含量测定。

Objective To develop a RP-HPLC method to detect the content of vitamin C in health foods and drinks. Method The samples were extracted and eluted by 0.1% oxalic acid solution on a ODS C18 5 μm column at the flow rate of 1.0 ml/min at 262 nm. Results The good linear relationship was obtained over the range of 0.05 - 700 μg/ml. The average recovery ranged from 84.2% to 94.9%, and the RSD of the values of repeatability was less than 10% . The detection limit of the method was 0.05 μg/ml. Conclusion The method could be simple, fast and feasible, and it could be used to detect the content of vitamin C in health foods and drinks.