内分泌腺体来源的血管内皮生长因子在子宫内膜异位症在位内膜及裸鼠模型中的特异性表达及意义

Specific expression and significance of endocrine gland-derived vascular endothelial growth factor in both eutopic endometrium and the nude mice model of endometriosis

目的探讨内分泌腺体来源血管内皮生长因子(endocrine gland-derived vascular endothelial growth factor,EG-VEGF)在子宫内膜及裸鼠模型表达及意义。方法留取异位症(EM组)和非异位症患者(NEM组)子宫内膜各24例,种植于裸鼠盆腹腔,5、15、30d处死,获取异位病灶。RT-PCR和原位杂交检测EG-VEGFmRNA表达。结果RT-PCR:2组各期异位病灶EG-VEGFmRNA表达均强于在位内膜,P<0.05,15d最强,与30d比较,P<0.05。原位杂交:2组在位内膜和异位病灶腺细胞、间质和血管内皮细胞均见EG-VEGFmRNA杂交信号,30天弱于5d及15d病灶。结论在位内膜也表达EG-VEGF,异位病灶EG-VEGF过表达,表达程度与异位内膜细胞活性相关。

[Objective] To explore the specific expression and significance of EG-VEGF （Endocrine Gland-derived Vascular Endothelial Growth Factor, EG-VEGF） in both eutopic endometrum and the nude mice model of en- dometriosis. [Methods] The late secretory endometria of 24 examples with endometriosis patients （EM group） and 24 examples without endometriosis （NEM group） were scraped and transplanted into the abdominopelvic cavity of the nude mice. The mice were killed on 5th d, 15th d and 30th d, then the implanted ectopic lesions were obtained. The expression of EG--VEGF mRNA in eutopic endometria and ectopic lesions of the nude mice were detected with RT- PCR and in situ hybridization. [Results] RT-PCR： The expression of EG-VEGF mRNA of all ectopic lesions was significantly higher than that in their corresponding eutopic endometria in both EM and NEM groups （P 〈0.05）, which the expression on 15th d was the strongest and there was significant difference compared with that on 30th d （P 〈0.05）. In situ hybridization： The positive expressive signals of EG-VEGF mRNA were seen in the endochylema of the glandular cells, interstitial and vascular endothelial cells in both eutopic endometria and ectopic lesions, and the expression of the lesions on 30th d was weaker than that on 15th d and 5th d. [Conclusion] The expression of EG-VEGF was also detected in eutoplc endometria. EG-VEGF expressed excessively in ectopic lesions in EM, and its expressive extent was correlative with the activity of ectopic cells.