EphB4和EphrinB2 mRNA在子宫颈癌组织中的表达及其与血管生成的关系

Expression of Eph B4 and Ephrin B2 mRNA in cervical cancer tissues and angiogenesis

目的检测EphB4和EphrinB2 mRNA在子宫颈癌组织中的表达,探讨其在子宫颈癌发生、发展及血管生成过程中的作用。方法采用逆转录多聚酶链反应(RT-PCR)法检测90例子宫颈癌、15例子宫颈上皮内瘤样病变(CIN)和15例正常宫颈组织中EphB4和EphrinB2 mRNA的表达,同时利用链霉菌抗生物素蛋白-过氧化酶连接(SP)法检测上述组织的微血管密度(MVD),分析EphB4和EphrinB2 mRNA的表达与各种临床病理参数和血管生成的关系。结果尽管所有子宫颈组织中均可检测出EphB4和EphrinB2 mRNA的表达,但是EphB4 mRNA在子宫颈癌(125.59±16.63)和CIN(122.61±17.30)组织中的表达强度均高于其在正常宫颈组织中的表达(95.72±4.63)(P均<0.05)。EphrinB2 mRNA在子宫颈癌组织中的表达强度(111.11±22.86)明显高于其在CIN(94.48±6.30)和正常宫颈组织中的表达(93.56±7.89)(P均<0.05)。EphB4 mRNA在子宫颈癌组织中的表达强度与子宫颈癌的临床分期和肿瘤直径密切相关(P均<0.05)。EphrinB2 mRNA在子宫颈癌组织中的表达强度只与子宫颈癌的肿瘤直径密切相关(P<0.05)。正常宫颈组织、CIN和子宫颈癌组织中MVD逐渐增加,分别为(11.33±2.38),(26.47±7.47)和(42.97±9.99)。子宫颈癌组织中EphB4和EphrinB2 mRNA的表达均与MVD呈正相关。结论EphB4和EphrinB2在子宫颈癌的发生、发展及血管生成过程中可能起重要的作用。

[ Objective] To detect the expression of EphB4 and EphrinB2 mRNA in cervical cancer tissues to probe their role in the oncogenesis, development and angiogenesis of cervical cancer. [Methods] The expression of EphB4, EphrinB2 mRNA and its microvessel density （MVD） in 90 patients with cervical caner, 15 patients with cer- vical intraepithelial neoplasm （CIN） and 15 patients with normal cervix were detected by reverse transcription-poly- merase chain reaction （RT-PCR） method and immunohistochemistry of streptavidin-peroxidase （SP） method. [ Resuits] Although Eph B4 and Ephrin B2 mRNA expression were detected in all tissues, the relative amount of Eph B4 mRNA was higher in cervical cancer （125.59±16.63） or CIN （122.61±17.30） than in normal cervices （95.72± 4.63） （P 〈0.05）. And the relative amount of Ephrin B2 mRNA was higher in cervical cancer （111.11±22.86） than in CIN （94.48±6.30） or normal cervices （93.56±7.89） （P 〈0.05）. In patients with cervical cancer, the relative amount of Eph B4 mRNA was correlated with both clinical stage and tumor diameter whereas the relative amount of Ephrin B2 mRNA was only correlated with tumor diameter. Values of MVD were found to increase gradually from normal cervix （11.33±2.38）, to CIN （26.47±7.47）, and to cervical cancer （42.97±9.99）. Relative amount of both Eph B4 and Ephrin B2 mRNA was shown to be positively eorrelated with MVD in patients with eervieal eaneer as well. [Conclusion] Eph B4 and Ephrin B2 interaetion may play an important role both in the oneogenesis and angiogenesis of eervieal eareinomas.