塞来昔布抑制SD大鼠膀胱癌作用的实验研究

Inhibition effect of celecoxib on SD mice bladder cancer

目的:探讨环氧化酶-2(Cox-2)的抑制剂塞来昔布(西乐葆,celecoxib)对N-丁基-N-(4-羟丁基)亚硝基胺(BBN)所诱导的SD大鼠膀胱癌抑制作用。方法:本实验将SD大鼠随机分成3组,均以BBN为致癌剂诱发SD大鼠膀胱肿瘤的生长。同时,其中的2个实验组另外喂以不同剂量的塞来昔布(0.5g/kg饮用水、1.0g/kg饮用水),30周后处死动物,观察塞来昔布对BBN所诱导的膀胱癌是否有抑制作用。结果:塞来昔布能明显抑制BBN所诱导的SD大鼠膀胱肿瘤结节的形成,P<0.05。塞来昔布的剂量越大,其对BBN所诱导的SD大鼠膀胱癌的抑制作用越强,P<0.05。结论:塞来昔布对BBN所诱导的SD大鼠膀胱癌有抑制作用。剂量较大的塞来昔布对BBN所诱导的SD大鼠膀胱癌的抑制作用更大。

OBJECTIVE： To detect whether cyclooxygenase-2 inhibitor celecoxib could prevent SD mice urinary bladder induced by N-butyl-N-（ 4-hydroxybutyl ）-nitrosamine （BBN）. METHODS： SD mice were divided into 3 groups randomly, and all the 3 groups were treated with BBN to induce urinary bladder cancer, and meanwhile 2 of the 3 groups were fed by celecoxib in addition at different dosages （0. 5 g celecoxib/kg tap water,1.0 g celecoxib/kg tap water）. The animals were sacrificed 30 weeks later to observe whether celecoxib could prevent SD mice urinary bladder cancer induced by BBN. RESULTS： Celecoxib obviously prevented SD mice urinary bladder tumour induced by BBN,P〈0. 05. The bigger dosage of celecoxib, the more potent preventive action against bladder cancer induced by BBN, P〈 0. 05. CONCLUSIONS： Celecoxib could prevent SD mice urinary bladder cancer induced by BBN. The bigger dosage of celecoxib, the more potent preventive action against bladder cancer induced by BBN.