三氧化二砷对白血病HL-60细胞株分化影响的研究

Effect of arsenic trioxide on leukemia cell line HL-60 differentiation

目的:探讨三氧化二砷(As2O3)治疗白血病的作用机制。方法:1)用流式细胞仪检测细胞膜上的CD11b的表达和细胞凋亡率。2)用RT-PCR方法检测c-myc基因在mRNA水平上的表达。结果:2.5μmol/LAs2O3处理HL-60细胞90h后,细胞分化的标志性细胞膜抗原CD11b表达明显升高,由(14.5±2.1)%升高到(35.4±5.7)%,P<0.05;NBT阳性细胞由(10.0±2.1)%升高到(31.2±3.4)%(P<0.05),同时c-myc基因在mRNA水平也明显降低。结论:As2O3通过降低c-myc基因表达及上调细胞分化抗原CD11b表达,从而促进HL-60细胞分化。

OBJECTIVE： To explore the mechanism of arsenic trioxide （As203）in the treatment of leukemia. METHODS： 1）The expression of CDllh, cell cycle and apoptosis were investi gated by flow cytometry. 2）The mRNA expression of c-myc was showed by RT PCR. RESULTS： Arsenic trioxide significantly increased CDllh in HL-60 cells, and the expression rate of CDllh increased from （14.5±2.1）% to （35.4±5.7）% after treated by 2.5 μmol/L arsenic trioxide for 90 hours （P（0. 05）, and the percentage of NBT positive cells increased from （ 10. 0±2. 1 ）% to （31.2±3.4） % （P〈0. 05）,and mRNA expression of c-myc was reduced significantly. CONCLUSION： Arsenic trioxide increases the cell differentiation in HL-60 cells by reducing mRNA expression of c-myc and increasing the expression of CDllb.