胸腺素α原作为乙型肝炎表面抗原佐剂的研究

Study of Prothymosin Alpha as Adjuvant to HBsAg

目的:研究胸腺素α原(ProTα)作为佐剂对重组乙型肝炎表面抗原(HBsAg)诱导小鼠产生乙型肝炎表面抗体(抗-HBs)的影响。方法:以纯系BALB/c小鼠为免疫对象,分组情况为:①HBsAg组(1μgHBsAg);②0.1μgProTα+1μgHBsAg组;③0.5μgProTα+1μgHBsAg组;④1μgProTα+1μgHBsAg组;⑤铝佐剂组(1μgVacon疫苗);⑥1μgPro-Tα+1μgVacon疫苗组;每组10只小鼠,分别于0、2周各肌肉注射免疫1次。采用ELISA法检测血清抗-HBs滴度(即IgG亚类IgG1和IgG2a)。结果:与单独注射HBsAg组相比,1μgProTα+1μgHBsAg组抗-HBs总抗体滴度明显增高(P<0.05),抗体持续时间更长,且ProTα可以平衡Th1和Th2免疫反应;2组间IgG1/IgG2a差异显著(P<0.05)。与铝佐剂相比,ProTα增强了小鼠对HBsAg的反应性,提高抗-HBs阳转率。结论:ProTα在增强小鼠抗-HBs产生的同时,提高细胞免疫反应,提示ProTα是一种很有潜力的HBsAg佐剂。

Objective：To investigate the effect of prothymosin α（ProTα） as adjuvant on anti-HBsAg immunoresponses in mice.Methods：The antigens were injected into the mice（BALB/c） twice i.m.at a interval of 14 days.The mice were divided into six groups with 10 mice in each group immunized with：①1 μg HBsAg;②0.1 μg ProTα＋1 μg HBsAg;③0.5 μg ProTα＋1 μg HBsAg;④1 μg ProTα＋1 μg HBsAg;⑤aluminum adjuvanted heptatis vaccine（1 μg Vacon vaccines）;⑥1 μg ProTα＋1 μg Vacon vaccines.The anti-HBs titers in sera（IgG and subclass IgG1 and IgG2a） were detected by ELISA.Results：The anti-HBs antibody titers from mice immunized with 1 μg ProTα＋1 μg HBsAg group were significantly higher than those of HBsAg group（P〈0.05）,and the antibody persistent time was potentially longer than HBsAg group.Prothymosin α made a better balance in Th1 and Th2 immune response,so the IgG1/IgG2a in 1 μg ProTα＋1 μg HBsAg group were significantly higher compared with HBsAg group（P〈0.05）.Compared with aluminum adjuvant,antibody responses to HBsAg were boasted by ProTα and the seroconversion rate was also improved.Conclusion：Both HBsAg specific humoral and cellular immuno-responses were boasted by ProTα in mice investigated,therefore ProTα was a potential adjuvant for HBsAg.