生物波调控因子对实验性脑梗死大鼠脑组织NF-κB表达的影响

The effect of Bio-wave regulation factor on nuclear factor-kappa B expression in rats after middle cerebral artery occlusion

目的:探讨生物波调控因子(BRF)对实验性脑梗死大鼠脑组织NF-κB表达的影响。方法:成年健康雄性SD大鼠90只随机假手术组、生理盐水组、BRF治疗组。制备大脑中动脉梗死(MCAO)模型,术后1h以1ml/100g的剂量分别腹腔注射1.25%BRF溶液和生理盐水,此后1次/d。进行行为学评分、干湿重法测定脑组织含水量、HE染色观察组织病理学改变、免疫组织化学方法测定脑组织NF-κB的表达。结果:(1)MCAO模型大鼠术后各时间点BRF治疗组的行为学评分较生理盐水组降低。(2)与假手术组比较,BRF治疗组和生理盐水组缺血脑组织含水量于24h开始明显升高,48h达高峰,持续至72h,7d明显下降。除6h外在各时间点均有显著性差异。BRF治疗组脑组织水肿程度减轻,48h脑含水量明显低于生理盐水组。(3)缺血区炎细胞浸润和NF-κB阳性细胞表达于梗死后6h开始增多,48h达高峰,持续至7d。假手术组未见明显炎细胞浸润,可见少量散在的NF-κB阳性细胞。BRF治疗组病理损伤减轻,术后48h、72h脑组织NF-κB阳性细胞表达明显低于生理盐水组。结论:生物波调控因子可以减轻梗死后脑水肿,降低脑组织NF-κB的表达,对大鼠脑组织的缺血损伤产生保护作用。

Objective：To investigate the effect of Bio-wave regulation factor（BRF） on nuclear factor-kappa B（NF-κB） expression in rats after middle cerebral artery occlusion（MCAO）.Methods：A total of 90 adult male Sprague-Dawley rats were randomly divided into sham operation group、normal saline group and BRF treatment group.Rats in BRF treatment group and normal saline group were subjected After MCAO and administrated intraperitoneally with 1.25 % BRF solution（1 ml/100 g） and normal saline（1 ml/100 g） respectively,1 h after operation,then once a day till they were sacrificed.Four different methods were used for assessing neurological deficit score（NDS）,the wet-dry method for measuring brain water content,hematoxylin and eosin staining technique for observing histopathological changes,immunohistochemistry method for detecting NF-κB expression.Results：（1） The NDS was lower in BRF treatment group than that of normal saline group at all time points.（2） Brain water contents both in normal saline group and BRF treatment group increased obviously at 24 h,reached to maximum at 48 h,continued to 72 h,and decreased remarkably at 7 d.There was significant difference between sham operation group and the other two groups at all time points except 6 h.Compared with normal saline group,brain edema in BRF treatment group was attenuated apparently,especially at 48 h.（3）The amounts of NF-κB positive cells increased at 6 h,peaked at 48 h,and continued to 7 d,as well as the inflammatory cell infiltration in the infarction regions.Scattered NF-κB positive cells were observed in sham operation group,without inflammatory cell infiltrating.Histopathologic damages were alleviated with BRF administration and the expression of NF-κB showed obviously decrease at 48 h、72 h.Conclusion：The results suggest that BRF can produce neuroprotective effects against infarction damage by reducing brain edema and inhibiting the expression of NF-κB.