BRF对局灶性脑梗死大鼠脑组织IGF-1表达的影响

The effect of BRF on IGF-1 expression after middle cerebral artery occlusion in rats

目的:探讨生物波调控因子BRF(Bio-wave regulationfactor)对实验性脑梗死大鼠脑组织中IGF-1表达水平的影响。方法:健康雄性SD大鼠90只,制成大脑中动脉梗死(MCAO)模型,随机分为3组,即BRF组、生理盐水和假手术组,各组又分为6,24,48,72h,7d5个亚组。BRF组为腹腔注射给药,生理盐水组给同体积的生理盐水,于相应时间点进行行为学评分、脑含水量及脑组织中IGF-1的表达。结果:①组织含水量:与假手术组相比,其他各组在各时间段均增加。其中术后48hBRF组低于同期的生理盐水组,P<0.05。②IGF-1阳性细胞表达:与假手术组相比,其他各组在各时间段均增加,梗死后6h开始增多,72h达高峰。术后72hBRF组高于同期的生理盐水组,P<0.05。结论:BRF可促进IGF-1表达,升高脑组织IGF-1含量,减轻局灶性脑梗死引起的缺血性损伤。

Objective：To investigate the effect of Bio-wave regulation factor（BRF）on insulin-like growth factor-1（IGF-1）expression in rats after middle cerebral artery occlusion（MCAO）.Methods：A total of 90 adult male Sprague-Dawley rats were subjected to MCAO,and they were randomly divided into sham operation group、normal saline group and BRF treatment group.Each group consisted of 5 subgroups（6 h、24 h、48 h、72 h and 7d）,according to different time points post-ischemia,The neurological deficit score（NDS）was assessed with 4 different methods,IGF-1 expression detected by immunohistochemistry method.Results：The amounts of IGF-1 positive cells increased at 6 h,with the peak at 72 h,and continued to 7 d,as well as the inflammatory cell infiltration in the infarction regions,There were several IGF-1 positive cells and no inflammatory cell infiltrating observed in the sham group.The NDS decreased and the histopathologic damages were alleviated with the BRF administration.The expression of IGF-1 was inhibited,significantly at 72 h（P〈0.05）.Conclusion：The results suggest that BRF can produce neuroprotective effects on the infarction damage by attenuating the brain edema and inhibiting the expression of IGF-1.