实验性自身免疫灰质病模型IgG的相关研究

Correlative study about IgG in experimental autoimmune gray matter disease model

目的:建立实验性自身免疫性灰质病(EAGMD)模型,通过观察IgG在神经肌肉接头(NMJ)处和运动神经元(MN)中的沉积情况,探讨IgG损伤MN的机制。方法:用牛脊髓免疫豚鼠建立EAGMD模型,对动物的临床表现,病理改变进行观察。结果:EAGMD动物临床表现为肌肉无力和萎缩;病理上出现腰髓运动神经元数量明显减少;IgG在NMJ处的沉积率明显增高(P<0.01),并且NMJ处IgG沉积率与动物病情进展程度有相关性,r=0.61。结论:EAGMD动物IgG可能通过NMJ参与MN的损伤。

Objective：The model of experimental autoimmune gray matter disease（EAGMD） was established in order to study the mechanism of the injury of the motoneurons by observing the aggradation of IgG on neuromuscular junctions（NMJ） and in motoneurons.Methods：EAGMD was established by guinea pigs be inoculated with the homogenite of bovine spinal,then the changes in clinic and pathology were observed.Results：In clinic,the weakness and atrophy of the muscles at EAGMD animals were found,the number of motor neurons in lumbar at EAGMD group was less;The ratio of IgG on Neuromuscular junctions at EAGMD was more than that at contral group（P〈0.01）,moreover,the correlation was found between the ratios of IgG on neuromuscular junctions and the developmental degree of the disaese at EAGMD,r=0.61.Conclusion：The injury of motoneuron at EAGMD animals may come from IgG by NMJ.