摘要
目的:探讨高功率微波(HPM)辐射对血睾屏障(BTB)结构与功能的影响。方法:166只二级雄性Wistar大鼠经0、10、30、100mW/cm2HPM辐射5min,于辐射后6h,1、3、7、14d,采用镧醛(含20g/L硝酸镧,50mg/L戊二醛)心脏灌注和2%伊文思蓝(EB)尾静脉注射,经光镜、电镜和激光共聚焦显微镜(LSCM)观察BTB结构变化及硝酸镧和EB分布。结果:10~100mW/cm2HPM辐射后大鼠睾丸间质水肿伴血管充血、出血;生精小管近腔室内血浆蛋白和红细胞积聚,病变在辐射后6h出现,1~7d明显加重,范围扩大,14d有所减轻;30、100mW/cm2辐射组明显重于10mW/cm2辐射组。电镜下见各辐射组硝酸镧颗粒于近腔室内精母细胞、精子细胞和精子之间沉淀。LSCM观察见生精小管近腔室内弥散分布的EB颗粒。结论:HPM辐射可引起BTB结构破坏,通透性增加。
Objective:To determine the effect of high power microwave(HPM)radiation on the structure and function of blood-testis barrier(BTB)in rats.Metiods:One hundred and sixty-six male Wistar rats were treated by heart perfusion of lanthanum-glutaraldehyde solution and tail vein injection of evans blue(EB)at 6 h,1,3,7 and 14 d after exposed to 0,10,30 and 100 mW/cm^2 HPM radiation for 5 minutes,the structural change of BTB and distribution of lanthanum or EB observed through the light microscope,electron microscope and laser scanning confocal microscopy(LSCM).Results:Testicular interstitial edema,vascular congestion or hyperemia with accumulation of plasma proteins and red blood cells in the inner compartment of seminiferous tubules were observed after exposure to HPM.The above-mentioned pathological changes were aggravated at 1-7 d and relieved at 14 d after radiation,obviously more severe in the 30 and 100 mW/cm^2 exposure groups than in the 10 mW/cm^2.Both lanthanum precipitation and EB were deposited in the inner compartment.Conclusion:HPM radiation may damage the structure and increase the permeability of BTB.
出处
《中华男科学杂志》
CAS
CSCD
2008年第7期579-582,共4页
National Journal of Andrology
基金
全军"十一五"医药卫生专项课题(06Z065)
关键词
高功率微波
血睾屏障
大鼠
high power microwave
blood-testis barrier
rat