高功率微波辐射对大鼠血睾屏障的影响

High Power Microwave Radiation Damages Blood-Testis Barrier in Rats

目的:探讨高功率微波(HPM)辐射对血睾屏障(BTB)结构与功能的影响。方法:166只二级雄性Wistar大鼠经0、10、30、100mW/cm2HPM辐射5min,于辐射后6h,1、3、7、14d,采用镧醛(含20g/L硝酸镧,50mg/L戊二醛)心脏灌注和2%伊文思蓝(EB)尾静脉注射,经光镜、电镜和激光共聚焦显微镜(LSCM)观察BTB结构变化及硝酸镧和EB分布。结果:10～100mW/cm2HPM辐射后大鼠睾丸间质水肿伴血管充血、出血;生精小管近腔室内血浆蛋白和红细胞积聚,病变在辐射后6h出现,1～7d明显加重,范围扩大,14d有所减轻;30、100mW/cm2辐射组明显重于10mW/cm2辐射组。电镜下见各辐射组硝酸镧颗粒于近腔室内精母细胞、精子细胞和精子之间沉淀。LSCM观察见生精小管近腔室内弥散分布的EB颗粒。结论:HPM辐射可引起BTB结构破坏,通透性增加。

Objective：To determine the effect of high power microwave（HPM）radiation on the structure and function of blood-testis barrier（BTB）in rats.Metiods：One hundred and sixty-six male Wistar rats were treated by heart perfusion of lanthanum-glutaraldehyde solution and tail vein injection of evans blue（EB）at 6 h,1,3,7 and 14 d after exposed to 0,10,30 and 100 mW/cm^2 HPM radiation for 5 minutes,the structural change of BTB and distribution of lanthanum or EB observed through the light microscope,electron microscope and laser scanning confocal microscopy（LSCM）.Results：Testicular interstitial edema,vascular congestion or hyperemia with accumulation of plasma proteins and red blood cells in the inner compartment of seminiferous tubules were observed after exposure to HPM.The above-mentioned pathological changes were aggravated at 1-7 d and relieved at 14 d after radiation,obviously more severe in the 30 and 100 mW/cm^2 exposure groups than in the 10 mW/cm^2.Both lanthanum precipitation and EB were deposited in the inner compartment.Conclusion：HPM radiation may damage the structure and increase the permeability of BTB.