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不同分化程度的胶质瘤细胞侵袭相关差异表达基因的筛选 被引量：2

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摘要 目的用基因芯片技术分析不同分化程度的胶质瘤细胞侵袭相关的差异表达基因,为进一步研究胶质瘤的侵袭机制提供依据。方法以Trizol试剂盒提取总RNA,用SuperscriptⅡ反转录酶进行反转录聚合酶链反应,并以荧光染料Cy3和Cy5分别标记胶质瘤细胞系SHG-44(WHOIV级)和CHG-5(WHOII级)的cDNA产物,然后进行芯片杂交,检测CHG-5和SHG-44细胞的基因表达谱,鉴定差异表达基因;随机选择数个差异基因进行Northern杂交实验,以验证cDNA微阵列结果的可靠性。结果与SHG-44相比,CHG-5细胞有102个基因表达上调、90个基因表达下调,这些差异表达基因按功能分为:凋亡类、细胞迁移和转移类、细胞周期与生长调节类、细胞骨架类、分化类、细胞代谢类、癌基因、氧化磷酸化类、受体与信号传导类、核蛋白体类、泛素-蛋白酶系统类、生长因子与细胞因子类及其他等。其中与侵袭相关的差异表达基因共13个(上调基因有6个、下调基因7个),芯片结果进一步得到Northern blot杂交结果的支持。结论初步揭示不同级别的胶质瘤细胞系之间存在侵袭相关基因的差异表达,提示这些差异基因可能与胶质瘤的进展有关。
作者 曾义 杨忠 韩杨云
机构地区 四川省德阳市人民医院神经外科 第三军医大学基础医学部
出处 《中国神经精神疾病杂志》 CAS CSCD 北大核心 2008年第7期428-430,共3页 Chinese Journal of Nervous and Mental Diseases
基金 四川省卫生厅科研项目(编号:050209)
关键词 胶质瘤 基因 CDNA 微阵列 侵袭
分类号 R739.4 [医药卫生—肿瘤]
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参考文献13

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中国神经精神疾病杂志
2008年 第7期

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