塞来昔布对胆管癌细胞株QBC939 VEGF表达的影响

Effects of Cyclooxygenase-2 Inhibitor Celecoxib on the Expression of VEGF in Human Cholangiocarcinoma QBC939 Cell Lines

目的探讨环氧合酶-2（COX-2）抑制剂塞来昔布对胆管癌细胞株QBC939血管内皮生长因子（VEGF）表达的影响。方法体外培养胆管癌细胞株QBC939，应用酶联免疫吸附试验（ELISA）和逆转录-聚合酶链反应（RT-PCR）方法检测塞来昔布与胆管癌细胞株QBC939VEGF表达间的时-效关系和量-效关系。结果塞来昔布在一定剂量（20-80μmol／L）和时间（12-48h）内显著抑制QBC939细胞VEGFmRNA的表达和培养上清液中VEGF的浓度，与单独培养液对照组比较，差别有统计学意义（P〈0．05）。结论选择性COX-2抑制剂塞来昔布可在一定时间和剂量范围抑制胆管癌细胞株QBC939VEGF的表达。

Objective To investigate the effects of the selective cyclooxygenase-2（COX-2） inhibitor, celecoxib, on the expression of vascular endothelial growth factor（VEGF） in human cholangiocarcinoma QBC939 cell lines. Methods The relationship between celecoxib and the expression of VEGF in human cholangiocarcinoma QBC939 cell lines were studied by using reverse transcription polymerase chain reaction（RT-PCR） and enzyme-linked immunoadsorbent assay （ELISA）. Results The expression of VEGF were inhibited significantly in a time-dependent（12 - 48h） and dose-dependent （20 - 80 μmol/L） manner than the control group（P〈0.05）. Conclusion The selective COX-2 inhibitor, celecoxib may play an important role on the inhibition of VEGF expression in human cholangiocarcinoma QBC939 cell lines.