姜黄素防治细菌脓毒症小鼠的实验研究

Preventive Effect of Cuncumin on Bacterial Sepsis Mice

目的探讨姜黄素对大肠埃希菌引起的细菌脓毒症小鼠的保护作用。方法昆明种小鼠90只随机分为对照组、模型组和姜黄素组,每组30只。姜黄素组腹腔注射姜黄素固体分散剂,每天1次,连续3d。第4天,模型组和姜黄素组尾静脉注射041184大肠埃希菌标准菌悬液建立细菌脓毒症模型。8h后,每组处死小鼠20只检测血清天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、肿瘤坏死因子α(TNF-α)和一氧化氮(NO)含量,观察肝、肾、肺和肠等脏器病理改变,余下小鼠观察24h生存率。结果姜黄素组血清AST、ALT、TNF-α和NO含量明显低于模型组(P<0.05),病理检查见脏器损害亦明显减轻;24h存活率90%,明显高于模型组(50%)。结论姜黄素可减少细菌脓毒症小鼠TNF-α、NO和氧自由基的产生,减轻其脏器病理损害。

Objective To investigate the effects of curcumin（Cur） on bacterial sepsis induced by Escherichia coli（E, coli） in mice. Methods Sixty mice were divided into 3 groups at random： a control group, an infected group and a curcumin group. Cur group, intraabdominal injection of curcumin solid dispersant（200 mg/kg） was given for three days. The infected group： 078 E. coli（5 10^10cfu/L, 10 mL/ kg） was administrated by intravenous injection to create the bacterial sepsis mice model. 8 hours after the injection, the level of AST, ALT, and TNF-α and NO of each group was measured and mice were sacrified to observe the pathological change of liver, ridney, lung and intestines. Results Curcumin can remarkably reduce the level of the AST and ALT in the serum of the bacterial sepsis mice induced by E. coli（P〈 0.05） as well as that of TNF-α（P〈0.05） and NO（P〈0.05）. Meanwhile, it can significantly decrease the pathological change of liver. Conclusion Curcumin can play an important role to protect the mice from bacterial sepsis by ways of cytotoxic effects of NO, TNF-α and oxygen free radicals.