微量元素氟对小鼠甲状腺功能的影响

Effect of fluorine on triiodothyronine and thyroxin in mice

目的:探讨微量元素氟及高氟补硒后对小鼠甲状腺功能的影响。方法:将80只小鼠随机分为一个对照组和三个实验组。预饲3天后,实验组分别腹腔注射20 mg/L(低氟组)、200 mg/L(高氟组)氟化钠溶液以及200 mg/L氟化钠与100 mg/L亚硒酸钠的混合溶液(高氟加硒组),对照组注射生理盐水。10天后和30天后各选取一半小鼠,观测其血清中T3、T4水平的差异,做短期研究和长期研究。结果:在短期影响中,低氟组的T3、T4水平与对照组比较有上升趋势,但无统计学意义(P>0.05);高氟组的T3、T4水平进一步上升,其中T4水平显著高于对照组,差异有统计学意义(P<0.05);高氟加硒组的T3、T4水平与对照组比较,差异无统计学意义(P>0.05),与高氟组比较呈下降的回复趋势。在长期影响中,低氟组的T3、T4水平与对照组比较有所下降,高氟组的T3、T4水平进一步下降,但均无统计学意义(P>0.05);高氟加硒组的T3、T4水平与高氟组比较又有所上升,但无统计学意义(P>0.05)。结论:高氟可影响小鼠甲状腺的分泌功能,短期使其分泌功能增强,长期有使其分泌功能减弱的趋势;高氟状态下补硒后,甲状腺功能的改变有回复趋势。

Objective： To investigate the influence of fluorine on the thyroid gland in mice. Methods： Eighty mice were randomly divided into control group and three experiment groups. The mice in control group,experiment groups with lower dose of fluorine and higher dose of fluorine group were injected physiological saline, NaF solution at 20 mg/L and 200 mg/L via peritoneal cavities, respectively. The mice in group with higher dose of fluorine mixed with selenium were injected solution containing NaF at 200mg/L and Na2SeO3 at 100 mg/L via peritoneal cavities. After ten days（short-term） and thirty days（long-term） ,the serum levels of T3 and T4 in the mice were measured. Results：The serum levels of T3 and T4 had no obvious difference between the group with a low dose of fluorine and control group（ P 〉 0.05 ）. The levels of T3 and T4 increased significantly in the group with high dose of fluorine and the level of T4 was much higher than that in the control group（ P 〈 0.05 ）. The levels of T3 and T4 had no significant differece between the group with higher dose of fluorine mixed with selenium and the control group, and the level was decreased compared with that of higher dose of fluorine group. Under long-term influence,the levels of T3 and T4 had no obvious difference between lower dose group and control group （P 〉 0.05）. Compared with control group,the level of T4 of higher dose group was higher（P 〈 0.05） ,while the level of T3 was lower, but the difference was not obvious（ P 〉 0.05 ）. The levels of T3 and T4 had no obvious difference between the group with higher dose of fluorine mixed with selenium and control group（ P 〉 0.05 ） Conclusions ： Fluorine at a high dose might interfere the secretive function of thyroid gland in mic^e,,It enhances the secretion of thyroid gland in short-term, but inhibits the secretive function in long-term. The changes of the secretive function of thyroid gland are correlative with the dose of fluorin and the time length of test. By supplying selenium,the secretive function of thyroid gland trends to come back to normal state from the disorder aroused by high dose of fluorin.