调气活血补肾汤对慢性肾功能不全大鼠的影响

Effect of Tiaoqi Huoxue Bushen decoction on experimental renal injury in rats

目的:观察调气活血补肾汤对慢性肾功能不全(CRF)大鼠肾组织匀浆NO及脂质过氧化的影响。方法:Wistar大鼠在乙醚麻醉下,切除5/6肾脏制备慢性肾功能不全模型。5周后,按小剂量(生药13.9 g/kg)、大剂量(生药27.8 g/kg)给模型大鼠灌胃给药,连续10周,于给药4周、10周后分两批处死大鼠,制备肾组织匀浆,测定NO、一氧化氮合酶(NOS)及超氧化物歧化酶(SOD)、丙二醛(MDA)含量。结果:成功复制大鼠CRF模型。CRF大鼠肾匀浆NO和NOS活性呈明显的早期升高、晚期下降趋势;使用调气活血补肾汤治疗4周时显著抑制NO和NOS活性过分明显升高,10周时显著抑制NO和NOS活性过分持续降低。随病程延长CRF大鼠肾组织SOD活性呈逐渐下降,MDA呈逐渐升高的趋势;调气活血补肾汤治疗后肾组织SOD活性明显增强,10周时显著下降,MDA则呈下降趋势;以上作用大剂量药物组均优于小剂量组。结论:调气活血补肾汤能改善和调节肾组织NO合成与释放、降低自由基损伤。

Objective：To observe the effect of Tiaoqi Huoxue Bushen decoction on the nitric oxide（NO） and lipid overoxidation of renal homogenate in rats with chronic renal failure （CRF）. Methods：The Wistar rats were resected five sixths of the kidney under anesthetization by ether to make animal models of chronic renal failure. After 5 weeks, the CRF rats were administered 13.9 g/kg BW and 27.8 g/kg BW for 10 weeks. All the rats were killed after 4 and 10 weeks, and the reanl homogenate was made. The relevant indexes such as NO, nitric oxide synthase （NOS） , superoxide dismutase （SOD） and malondialdehyde （MDA） were measured in the reanl homogenate. Results：The model of CRF rats was successfully established. The activity of NO and NOS in the reanl homogenate of CRF rats rose obviously at the early stage and descented at the later period . The over-rise and persistent decrease of the activity of NO and NOS were obviously inhibited after the administration of Tiaoqi Huoxue Bushen decoction for 4 weeks and 10 weeks, respectively. With the prolonging of the desease ,the SOD activity in the nephridial tissue of the CRF rats kept decreasing, and the content of MDA kept decreasing. After the therapy of Tiaoqi Huoxue Bushen decoction, the SOD activity and the content of MDA in the nephridial tissue of the CRF rats improved significantly. The effect was more obvous in the group treated with a large dose than in that receiving a small dose. Conclusions：The results demonstrate that Tiaoqi Huoxue Bushen decoction can improve and adjust the synthesis of NO, and decrease the free radical injury as well.