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1,25-二羟维生素D_3联合塞莱昔布对乳腺癌细胞株Hs578T生长及凋亡的影响 被引量：1

The Effect of Combination Use of 1,25(OH)_2D_3 and Celecoxib on Growth and Apoptosis in Breast Cancer Cell Line Hs578T
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摘要 目的研究1,25-二羟维生素D31,25(OH)2D3联合塞莱昔布对乳腺癌细胞株Hs578T生长、细胞周期及凋亡的影响。方法采用四唑氮蓝比色(MTT)法检测细胞增生,流式细胞仪检测细胞周期及凋亡率。结果1,25(OH)2D3及塞莱昔布均可抑制乳腺癌细胞增生,诱导细胞凋亡,呈时间与剂量依赖性。当联合塞莱昔布时抑瘤率较1,25(OH)2D3单药具有叠加作用,但低于塞莱昔布单药。10-8mol/L联合塞莱昔布组抑瘤率高于10-7mol/L联合塞莱昔布组。结论1,25(OH)2D3及塞莱昔布可成为新一类乳腺癌预防、治疗药物。 Objective To study the effect of combined 1,25 -dihydroxyvitamin D3 [1,25 （OH）2 D3] and celecoxib on growth, call cycle and apoptosis in breast cancer cell line Hs578T. Methods We compared cell numbers by using MTT method , analyzed cell cycle percentage and apoptosis with flow cytometric. Results Both with 1,25 （OH）2 D3 and celecoxib could inhibite tumor growth ,indue apoptosis in a dose-time -dependent manner, comparing with 1,25 （OH）2 D3 alone, combination wse of the two drugs had a additive effect but was inferior to cececoxib alone. The combination use of 10 μmol/L1,25 （OH）2D3 and celecoxib group is more effective than the combination use of 10^-7μmoL/L1,25 （OH） 2 D3 and celecoxib group. Conclusion 1,25 （ OH ）2D3 and celecoxib could be a new drug for the prevention and treatment of breast cancer.
作者 何雪琦 鲍扬漪 孙昕 孟刚 汪勤
机构地区 安徽省合肥市第一人民医院肿瘤科
出处 《医学研究杂志》 2008年第8期66-68,共3页 Journal of Medical Research
基金 合肥市科技局重点科研项目(合科2007第15号)
关键词 1 25-二羟维生素D3 塞莱昔布 凋亡 细胞增生 1,25-dihydroxyvitamin D3 Celecoxib Apotosis Cell proliferation
分类号 R977.24 [医药卫生—药品] R737.9 [医药卫生—肿瘤]
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参考文献5

  • 1Smith DC, Johnson CS, Freemen CC, et al. A Phase I trial of calcitriol ( 1,25 - dihydroxycholecalciferol) in patients with advanced malignancy. Clin Cancer Res, 1999,5:1339 - 1345
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同被引文献18

  • 1Niu Guangfeng,Liao Zhichao,Cai Lin,et al.The Combined Effects of Celecoxib and Minocycline Hydrochloride on Inhibiting the Osseous Metastasis of Breast Cancer in Nude Mice[J].Cancer Biotherapy & Radiopharmaceuticals,2008,4(23):469-476.
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  • 4Tian Xiaoxiao,Du Hao,Fu Xiangsheng,et al.Smad4 restoration leads to a suppression of Wnt/β-catenin signaling activity and migration capacity in human colon carcinoma cells[J].Biochemical and Biophysical Research Communications,2009,380:478-483.
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  • 7Peng Lihong,Malloy P J,Feldman D.Identification of a Functional Vitamin D Response Element in the Human Insulin-Like Growth Factor Binding Protein-3 Promoter[J].Molecular Endocrinology,2004,18(5):1109-1119.
  • 8Shimizu M,Deguchi A,Hara Y,et al.EGCG inhibits activation of the insulin-like growth factor-1 receptor in human colon cancer cells[J].Biochemical and Biophysical Research Communications,2005,334:947-953.
  • 9Maier T J,Janssen A,Schmidt R,et al.Targeting the beta-catenin/APC pathway:a novel mechanism to explain the cyclooxygenase-2-independent anticarcinogenic effects of celecoxib in human colon carcinoma cells[J].The FASEB Journal,2005,9:1-25.
  • 10Yasumaru M,Tsuji S,Tsujii M,et al.Inhibition of Angiotensin II Activity Enhanced the Antitumor Effect of Cyclooxygenase-2 Inhibitors via Insulin-Like Growth Factor I Receptor Pathway[J].Cancer Research,2003,63:6726-6734.

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医学研究杂志
2008年 第8期

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