钦州湾牡蛎线粒体16 S rRNA基因片段核苷酸序列分析

Study on the Single Nucleotide Polymorphism in Populations of Oyster Mitochondrial 16 S rRNA Gene Fragment in Qin Zhou Gulf

对69个钦州湾牡蛎个体的线粒体DNA16S rRNA进行PCR扩增,纯化后的PCR产物测序分析,研究16S rRNA基因在白肉牡蛎和红肉牡蛎2个群体中的遗传多样性,通过DNAman比对序列并构建系统进化树。结果得到2个群体的序列长度均为434bp,共检测到16个核苷酸突变位点,包括8个转换位点和8个颠换位点。与GenBank序列比对发现,白肉牡蛎和香港牡蛎的序列基本相同,系统进化树中显示白肉牡蛎与香港牡蛎聚为一支,红肉牡蛎与有明巨牡蛎聚为一支。证实了白肉牡蛎和红肉牡蛎是2个不同的种,它们有各自的遗传多样性,该研究为牡蛎的遗传育种提供科学依据。

PCR technique was used to amplify the mtDNA 16 S rRNA gene fragment in 69 individuals of oyster from Qin Zhou Gulf population. The purified PCR products were sequenced and analyzed to study the genetic polymorphism of 16 S rRNA gene in two species of white meat oysters and red meat oysters, Sequences were aligned and the phylogenetic tree were built using DNAman. As a result, the length of sequence in two species were 434 bp, 16 nucleotide mutant sites were detected, including eight transition sites and eight transversion sites. The sequence of white oyster was essential same to C. hongkongensis by compared with GenBank, the phyiogenetic tree showed that white oyster congregated with C. hongkongensis, red oyster congregated with C. ariakensis. In conclusion,white oyster and red oyster were two different species, they had respectively differential genetic polymorphism, the research provided science foundation for oyster＇s genetic breeding.