HGF、CXCR-4、VEGF-D表达与甲状腺乳头状癌颈淋巴结转移的关系

Relation between expression of HGF,CXCR-4,VEGF-D and the lymph node metastasis in papillary thyroid carcinoma (PTC)

目的:通过检测HGF、CXCR-4、VEGF-D蛋白在甲状腺乳头状癌(papillary thyroid carcinoma,PTC)中的表达差异,探讨三种因子与PTC淋巴结转移的关系。方法:随机选取有完整病例记录和追踪观察的乳头状癌病例70例,采用免疫组化ABC法,研究HGF、CXCR-4、VEGF-D等基因产物在PTC的表达情况。结果:70例PTC病人中,三种因子在有淋巴结转移的阳性表达率分别为91.30%、71.74%和89.13%,三种因子在无淋巴结转移组的阳性表达率分别为66.67%、37.50%和37.50%,有淋巴结转移组的阳性表达率均高于无淋巴结转移组,差异有统计学意义;在70例PTC病人中,VEGF-D和CXCR-4表达均为阳性的有39例,二者在PTC中的表达有显著相关性,(相关系数r=0.502,P<0.05)。结论:HGF、CXCR-4可能影响PTC癌细胞的迁移能力而引起淋巴结转移;VEGF-D通过诱导淋巴管内皮细胞新生和淋巴管生成,从而促进PTC淋巴结转移;VEGF-D促进PTC淋巴结转移的作用可能需趋化因子CXCR-4协同参与。

Objective ： To study the expression level of 3 factors （ CXCR - 4, HGF, VEGF - D） and the correlation between the 3 factors and lymph node metastasis in PTC. Methods： Total of 70 cases of PTC with complete clinical records and preliminary diagnosis were picked out from patients in Tianjin Cancer Hospital. The expression of HGF, CXCR - 4, VEGF - D in 70 cases of PTC were assayed by using immunohistochemical staining. Results： In 70 cases of PTC,the rate of immuno - positive staining HGF CXCR -4 VEGF - D were 91.30% ,71.74% ,89. 13% with lymph node metastasis and 66.67%, 37.50%, 37.50% without metastasis respectively, （ P 〈 0.05 ） ; The rates of simultaneous immuno - positive staining of VEGF - D and CXCR -4 in 70 papillary thyroid carcinoma were 55.71% （39/70）. The positive relationship has been found between the expression status of VEGF - D and CXCR - 4 and cervical lymph node metastasis. Conclusion： HGF and CXCR-4 might be useful to promote lymph node metastasis of PTC by affecting the migration ability of carcinoma ; VEGF - D might cause lymph node metastasis of PTC by lymphangiogenesis ;The expression of VEGF- D is correlated with the expression of CXCR- 4 in PTC, the patients with VEGF - D positive and CXCR -4 positive had high possibility of lymph node metastasis.