巯嘌呤分子探针同步荧光法测定蛋白质的研究

Determination of protein by synchronous fluorescence as 6-mercaptopurine molecular probe

基于巯嘌呤与血清白蛋白相互作用引起血清白蛋白的同步荧光发生特异性变化，其同步荧光强度和溶液中血清白蛋白的浓度呈良好的线性关系，建立了以巯嘌呤为分子探针，运用固定波长同步荧光光谱分析测定生物样品中蛋白质含量的新方法。在最佳实验条件下，体系的同步荧光强度与人血清白蛋白在0．55—496．8μg／mL范围内呈良好的线性关系，方法的检测限可达0．18μg／mL。方法简便快速，灵敏度高，线性范围宽，稳定性及选择性好，对实际样品进行回收测定，回收率在97．9％-102．7％之间，对11份空白溶液进行平行测定的相对标准差为0．86％。

6-Mercaptopurine can interact with serum albumin, resulting in the specific changes of the endogenous fluorescence of serum albumin. Synchronous fluorescence intensity of this system has a good linear relationship with the concentration of serum albumin in solution. A new method for determining the protein in serum samples was established according to the principium described above. Under the optimum experimental conditions, human serum albumin has a good hnear relationship in the range of 0.55 - 496.8 μg/mL and the detection limit is 0.18 μg/mL.The method is simple and rapid. Eleven blank solutions for conducting the parallel experimental study obtained a relative standard deviation of 0.86% and the recovery was 97.9% - 102.7% .The results showed that 6-mercaptopurine as a molecular probe used in synchronous fluorescence spectroscopy method could resulted in a simple and rapid method with high sensitivity, featuring wide linear range of determination, stability and good selectivity.