^(99m)Tc-HL91显像监测荷瘤鼠高压氧后乏氧状况

Study of ^(99m)Tc-HL91 imaging to monitor hypoxiain mice tumor after hyperbaric of oxygen

目的99mTc-HL91乏氧显像评价肿瘤高压氧后的乏氧状况。方法20只荷H22肝癌KM小鼠随机分为4组,每组5只;(1)对照组,只进行99mTc-HL91乏氧显像;(2)15 min组,出高压氧舱后15 min行99mTc-HL91乏氧显像;(3)30 min组,出高压氧舱后30 min行99mTc-HL91乏氧显像;(4)60 min组,出高压氧舱后60 min行99mTc-HL91乏氧显像。在平面图像上,通过核医学感兴趣区(regionfinteresting,ROI)技术,计算肿瘤与对侧相应部位放射性比值(T/NT),图像采集结束后立即处死小鼠,完整剥离肿瘤,称重并计算放射性计数,计算肿瘤微分摄取率(DUR)。结果15 min组、30 min组DUR及T/NT与对照组比较,差异有统计学意义(P<0.05),60 min组与对照组比较,差异无统计学意义(P>0.05)。结论99mTc-HL91显像是一种有价值的评价肿瘤高压氧后乏氧状况的方法。

Objective To evaluate whether ^99mTc-HL91 hypoxia imagining could monitor the status of tumor hypoxia after hyperbaric oxygen. Methods Twenty bearing 1-122 tumor KM mice were randomly divided into 4 groups, 5 mice/group： The control group： only to carry on ^99mTc-HL91 hypoxia imaging. The 15 minute group, to carry on ^99mTc- HL91 hypoxia imaging at 15 minute after hyperbaric oxygen. The 30 minute group, to perform ^99mTc-HL91 hypoxia imaging at 30 minute after hyperbaric oxygen. The 60 minute group, to perform ^99mTc-HL91 hypoxia imaging at 60 minute after hyperbaric oxygen. ROI were drawn around tumor and contralateral muscles in planar images to calculate the T/NT, then the tumor was excised, weighted and the radioactivity was measured. Differential uptake ratio（DUR） was computed to quantitate the tracer uptake. Results Compared with the control group, the tumor DUR and T/NT of 15 minute group and 30 minute group were significantly different （ P 〈 0.05）, 60 minute group suggested no significant difference（ P 〉 0. 05）. Conclusion The ^99mTc-HLgl hypexia imaging might be valuable in evaluating the status of tumor hypoxia after hyperbaric oxygen.