糖蛋白的凝胶电泳和电印迹染色鉴定技术

Techniques for Staining Glycoprotein on Gel Electrophoresis or Electroblotting

糖基化是蛋白质翻译后修饰最重要的方式之一,是必需的生理过程,对蛋白质生物学功能的正确发挥起着至关重要的作用。二维凝胶电泳(two-dimensional gel electrophoresis,2-DE)是常用的分离蛋白质的方法之一,糖蛋白在凝胶上的检测是分离鉴定糖蛋白的关键之一。本文对近年来凝胶电泳、电印迹技术中鉴定糖蛋白的衍生试剂的研究和应用进行了综述,对多种衍生试剂衍生糖蛋白的机理作了介绍。

Glycosylation is one of the most important protein post-translational modification methods, which is essential to life and function of proteins. Two-dimensional gel electrophoresis （2-DE） is one of the most commonly used methods developed for the separation of proteins, and identification of glycoproteins in gel is a key step for the separation and purification of glycoproteins. This review gives an overview of derivatization reagents for detecting glycoproteins on gel electrophoresis or electrohlotting, and the reaction mechanisms of several derivative reagents are also introduced.