激活蛋白-2对Hela细胞中survivin的抑制作用

Activator protein-2 inhibits survivin gene expression in Hela cells

目的探讨激活蛋白-2(AP-2)对Hela细胞中survivin基因表达的影响,为靶向抑制survivin基因表达的肿瘤治疗奠定理论和临床应用基础。方法共转染survivin启动子-荧光素酶报告基因质粒和AP-2,双荧光素酶活性检测AP-2对survivin启动子活性的影响;转染AP-2到Hela细胞中,反转录-聚合酶链反应(RT-PCR)和Westernblot检测AP-2对Hela细胞中survivin基因表达的影响。结果AP-2能抑制survivin核心启动子活性;并在转录和翻译水平上抑制Hela细胞中的survivin基因表达。结论AP-2可抑制Hela细胞中survivin基因的表达,为有效控制survivin基因表达提供了新的思路,为靶向survivin的肿瘤基因治疗奠定基础。

Objective To elucidate the impacts of activator protein-2 （AP-2） on survivln gene expression in Hela cells and to provide a theoretical and clinical basis for targeted suppression of survivin gene. Methods The survivin promoter and AP-2 were eotransfeeted into Hela cells to detect the impacts of AP-2 on activities of survivin promoter. The regulation of the survivin expression by AP-2 was detected with RT-PCR and Western blotting after transfection. Results AP-2 was shown to inhibit the activity of survlvin core promoter in Hela cells. In addition, AP-2 also inhibited survivin expression at the transcription and translation levels in Hela cells. Conclusion Inhibition of survivin expression in Hela cells by AP-2 may open up a new approach for effective regulation of survivin gene and provide a potential base for survivin-targeted cancer therapy.