仙客来愈伤组织的诱导及其抗褐变的研究

Study on Callus Inducement and Anti-browning of Cyclamen persicum Mill

该试验以仙客来的叶片、叶柄、花梗为外植体,分别以MS为基本培养基,并添加了不同种类和浓度的植物生长激素,对其进行愈伤组织的诱导。另外,还初步研究了活性炭(AC)、VC、聚乙烯吡咯烷酮(PVP)和黑暗培养对仙客来外植体抗褐变的作用。结果表明,以叶片为外植体诱导效率最高,叶柄、花梗次之。叶片愈伤组织诱导的最适培养基为1/2MS+6-BA2.0mg/L+2,4-D0.5mg/L+KT0.25mg/L;叶柄愈伤组织诱导的最适培养基是MS+6-BA0.2mg/L+NAA2.5mg/L;花梗愈伤组织诱导的最适培养基是MS+6-BA0.2mg/L+2,4-D0.5mg/L。5种抗褐化处理方法中黑暗培养抗褐化效果最好。

The leaves, leafstalk and peduncles of Cyclamen persicum Mill were taken as expand in this experiment, and they were cultured on MS basic medium respectviely with different kind of phytohormones among various concentrations. The influences of phytohormones on different explants and the role of active carbon （AC）, vitamin c （VC）, polyvinylpyrrolidone （PVP） and darkness culture in anti-browning of explants were studied in this experiment by inducing the callus in different medium. The results showed that the best explants for inducing callus was young leaves, leafstalks and peduncles took the second place. The best medium for callus induction of leaves, leafstalks and peduncles were 1/2MS＋6-BA2.0mg/L＋2,4-D0.5mg/L＋KT0.25mg/L MS＋6-BA0.2mg/L＋ NAA2.5mg/L, and MS＋6-BA0.2mg/L＋2,4-D0.5mg/L respectively. Darkness culture was best for anti-browning among the five treatments.