一株降解苯酚微生物的分离与鉴定

Isolation and Identification of A Phenol-degrading Bacterium

从武汉市某化工厂的活性污泥中分离1株能以苯酚为惟一碳源和能源生长的菌株,命名为CY1。通过逐级驯化,CY1可在含1 000 mg·L-1苯酚的培养基中降解苯酚并生长。经对该菌株进行形态特征以及16S rDNA序列比对分析,确认该菌株属于假单胞菌属(Pseudomonas)。

A strain CY1 was isolated from the sludge around a chemical plant in Wuhan, Hubei Province. It had ability of utilizing phenol as sole carbon and energy sources. By using step-by-step domesticating method, it could grow in the culture medium byusing 1 000 mg·L^-1 phenol as sole carbon source, CY1 was considered to be belonged to Pseudornonas by morphological characteristics and the phylogenetical analysis of the 16S rDNA sequence.