全反式维甲酸对小细胞肺癌细胞的分化诱导作用研究

Differentiation and induction of the small cell lung cells with all-trans-retinoic acid

目的探讨全反式维甲酸(all-trans-retinoic acid,ATRA)对小细胞肺癌细胞的分化诱导作用。方法选择NCI-H446细胞株MTT法检测细胞体外增殖能力,电镜下观察形态,流式细胞仪进行细胞周期分析。RT-PCR法分析维甲酸受体亚型基因。结果全反式维甲酸诱导小细胞肺癌NCI-H446细胞株,细胞增殖能力下降,凋亡增加,更多的细胞被阻止于G1/G0期。结论全反式维甲酸诱导分化能有效抑制小细胞肺癌NCI-H446细胞株的增殖,促进凋亡。

Objective To study the differentiation and induction of the small eeU lung cells with all-trans-retinoie acid （ATRA）. Methods The proliferation and differentiation of NCI-H446 cells were examined by the methods of MTT. Electron microscope examination was applied to viewing cell morphological change, flow cytometry performing cell cycle analysis, and RT-PCR assay was adopted to detect the expression of retinoic acid receptor subgroup gene （RARs and RXRs）. Results ATRA could obviously inhibit the growth and the differentiation of NCI-H446 cells and more cells underwent apoptosis and were arrested in G1/G0 phase. Conclusion ATRA can inhibit the differentiation and proliferation of small cell lung cancer NCI-H446 cell line and promote its apoptosis.