小麦杂交后代的高分子量麦谷蛋白亚基组成分析

Composition Analysis of High-molecular-weight Subunits of Glutenin of Wheat Hybridization Progenies

以郑州9023和野二二燕4 F杂交F2代株系为试材,用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)法鉴定分析了杂交后代的高分子量(HMW)麦谷蛋白亚基组成。结果表明,在Glu-A1、Glu-B1和Glu-D1三个位点上分别检测到2、3和1种亚基类型,3位点结合共出现3种组合类型。其中,在Glu-A1位点上出现了1和N亚基,1优质亚基出现的频率高达66.7%;在Glu-B1位点上,13+16亚基出现频率最高(55.6%);Glu-D1位点上出现的全是被世界公认的优质亚基5+10。1、13+16、5+10亚基组合类型出现的频率最高(55.6%),该类型兼有1和5+10优质亚基。

The compositions of high-molecular-weight （HMW） subunits of glutenin of the F2 progenies derived from Zhengzhou 9023 × Yeereryan 4 F were researched using sodium dodecyl sulphate-polyacrylamide gel electrophoresis （SDS-PAGE） method in the present study. The results showed that two, three and one HMW subunit types of glutenin were found in the loci of Glu-A1, Glu-B1 and Glu-D1, respectively, and three composition types appeared. For Glu-A1, subunits 1 and N occurred, and the frequency of high quality subunit 1 was as high as 66.7% ;for Glu-B1, the frequency of subunit pair 13 ＋ 16 was the highest （55.6%） ; for Glu-D1, only subunit pair 5 ＋ 10 （regarded as the highest quality subunit pair in the world） appeared. The frequency of composition type 1, 13 ＋ 16 and 5 ＋ 10 was the highest （55.6%）, which contained high quality subunit 1 and subunit pair 5 ＋ 10.