不同产地鱼腥草中绿原酸、槲皮素含量测定

Determination of chlorogenic acid and quercetin in Houttuynia cordata Thunb.from different districts

目的:建立高效毛细管电泳法(HPCE),测定不同产地鱼腥草中绿原酸和槲皮素的含量。方法:毛细管电泳柱为未涂层弹性石英毛细管(60.5 cm×75 μm),运行缓冲液为 pH 8.5的30 mmol·L^(-1)硼砂体系,分离电压16 kV,检测波长为254 nm,柱温为25℃。结果:2种物质在14 min 内得到了良好分离。绿原酸和槲皮素浓度分别在0.05～0.8 mg·mL^(-1)和0.04～0.15mg·mL^(-1)范围内与峰面积呈良好的线性关系,平均加样回收率分别为98.7%和98.8%,RSD 分别为2.2%和2.5%(n=3)。结论:此法操作简便快捷,回收率及重复性好,可作为鱼腥草质量控制的方法。

Objective：To establish a high - preformance capillary electrophoresis （HPCE） method for determination of chlorogenic acid and quercetin in Houttuynia cordata Thunb. from different districts. Methods：The capillary pillar used in this study was capillary without smear layer, the buffer solution was pH 8.5 and 30 mmol · L^-1 sodium borate solution, the running voltage was 16 kV, the wavelength of detection was 254 nm, and the column temperature was 25 ℃. Results：The detected concentration of chlorogenic acid and quercetin showed good linear relationships with the peak area in the ranges of 0.05 - 0. 8 mg ·mL^-1 （ r = 0. 9995 ） and 0.04 - 0. 15 mg ·mL^-1 respectively. The average recoveries were 98.7% and 98.8% , with RSD of 2.2% and 2.5% （n = 3）. Conclusion：The method is simple, rapid, and good reproducibilities. It can be used to control the quality of Houttuynia cordata Thunb.