摘要
目的:建立高效毛细管电泳法(HPCE),测定不同产地鱼腥草中绿原酸和槲皮素的含量。方法:毛细管电泳柱为未涂层弹性石英毛细管(60.5 cm×75 μm),运行缓冲液为 pH 8.5的30 mmol·L^(-1)硼砂体系,分离电压16 kV,检测波长为254 nm,柱温为25℃。结果:2种物质在14 min 内得到了良好分离。绿原酸和槲皮素浓度分别在0.05~0.8 mg·mL^(-1)和0.04~0.15mg·mL^(-1)范围内与峰面积呈良好的线性关系,平均加样回收率分别为98.7%和98.8%,RSD 分别为2.2%和2.5%(n=3)。结论:此法操作简便快捷,回收率及重复性好,可作为鱼腥草质量控制的方法。
Objective:To establish a high - preformance capillary electrophoresis (HPCE) method for determination of chlorogenic acid and quercetin in Houttuynia cordata Thunb. from different districts. Methods:The capillary pillar used in this study was capillary without smear layer, the buffer solution was pH 8.5 and 30 mmol · L^-1 sodium borate solution, the running voltage was 16 kV, the wavelength of detection was 254 nm, and the column temperature was 25 ℃. Results:The detected concentration of chlorogenic acid and quercetin showed good linear relationships with the peak area in the ranges of 0.05 - 0. 8 mg ·mL^-1 ( r = 0. 9995 ) and 0.04 - 0. 15 mg ·mL^-1 respectively. The average recoveries were 98.7% and 98.8% , with RSD of 2.2% and 2.5% (n = 3). Conclusion:The method is simple, rapid, and good reproducibilities. It can be used to control the quality of Houttuynia cordata Thunb.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第7期1081-1083,共3页
Chinese Journal of Pharmaceutical Analysis
基金
安徽省优秀青年基金资助项目(06041083)
安徽省高校"十五"优秀人才资助项目
关键词
高效毛细管电泳
鱼腥草
绿原酸
槲皮素
HPCE
Houttuynia cordata Thunb.
chlorogenic acid
quercetin