民族药三姐妹的质量研究

Study on quality study of Rabdosia temifolia(D.Don)Hara(Sanjiemei)

目的:建立三姐妹药材的 TLC 鉴别法以及药材中齐墩果酸和熊果酸的 HPLC-ELSD 含量测定法,考察广西不同产地、不同药用部位的药材质量。方法:以齐墩果酸为指标成分,三氯甲烷-甲醇(35:1)为展开剂,采用 TLC 法对三姐妹药材进行定性;以 Shim-pack VP-ODS(4.6 min×250 mm,5 μm)为色谱柱,甲醇-0.5%醋酸铵(85:15)为流动相,流速1.0 mL·min^(-1),Alltech ELSD 2000蒸发光散射检测器,采用 HPLC-ELSD 法对药材中齐墩果酸和熊果酸进行定量。结果:TLC 斑点分离清晰,简便易行;齐墩果酸和熊果酸线性范围分别为0.42～2.5μg(r=0.9998)和0.48～2.9μg(r=0.9997)。加样回收率齐墩果酸为98.3%(RSD=1.4%),熊果酸为100.3%(RSD=1.7%)。结论:本方法简便、准确,重复性好,专属性强,可用于该药材的质量控制和评价。

Objective ： TLC and HPLC - ELSD methods were established to investigate the quality of Rabdosia temifolia（ D. Don）Hara in Guangxi with different habitats and officinal parts. Methods ：oleanolic acid as target constituent,Rabdosia temifolia（ D. Don）Hara was identified by TLC ,used the chloroform-methanol（35：1 ）as developing agent ; and the determination of oleanolic acid and ursolic acid were conducted by HPLC - ELSD with a Shim - pack VP - ODS （4. 6 mm × 250 mm,5 μm） column. The mobile phase was methanol - 0. 5 % ammonium acetate solution （85：15 ） ,at a flow rate of 1.0 mL · min^-1, and the detector was Alltech ELSD 2000. Results：The TLC method was easy and the spots were separated clearly;the calibration curves of oleanolic acid and ursolic acid were linear in range of 0. 42 - 2.5 μg （ r = 0. 9998 ） and 0. 48 - 2. 9μg （ r = 0. 9997 ）, respectively. The average recoveries of oleanolic acid and ursolic acid were 98.28% （ RSD = 1.4% ） and 100. 3% （ RSD = 1.6% ）, respectively. Conclusion ：The developed method is simple, accurate, reproducible, specific and can be used for the quality control of Rabdosia temifolia （ D. Don） Hara.