738例汉族儿童甘露聚糖结合凝集素血清水平测定

Determination of the serum mannose binding lectin levels in 738 Han ethnic group children

目的通过检测健康新生儿脐带血、正常儿童以及成人血清甘露聚糖结合凝集素（MBL）水平，了解汉族血清MBL的分布范围。方法采用酶联免疫吸附试验（ELISA）检测重庆、武汉、乌鲁木齐268例汉族足月顺产健康新生儿脐带血、重庆地区470例0-6岁正常儿童血清MBL水平，并以87例成人作为对照。结果儿童（28d-6岁）血清MBL值呈非正态分布，且无年龄、性别差异。根据不同年龄组分析，新生儿血清（884—1825μg／L，中位数1597μg／L）、新生儿脐带血的MBL水平最低（0—4604μg／L，中位数1462μg／L），与其他年龄组（儿童组0—7860μg／L，中位数2536μg／L；成人组98—6495μg／L，中位数2920μg／L）比较差异有统计学意义（P〈0．01）。而28d-组（214—4195μg／L，中位数2299μg／L）、6个月-组（5—4637μg／L，中位数2622μg／L）及2-6岁组（198—7860μg,／L，中位数2585μg,／L）组间MBL水平比较差异无统计学意义（P〉0．1）。分析28d-6岁儿童血清MBL水平数据得出中位数为2563μg／L，P2.5-P97.5为171—5079μg／L。结论28d-6岁儿童血清MBL水平呈非正态分布，无性别、年龄差别；28d-6岁的正常儿童血清MBL水平95％的可信区间为171—5079μg／L。

Objective To investigate the distribution of serum mannose binding lectin （MBL） levels in Han ethnic group children. Methods The concentrations of MBL in serum were measured by ELISA in 268 umbilical cord blood specimens from Chongqing, Wuhan and Urumqi as well as in serum of 470 normal children aged from 0 to 6 years and 87 adults in Chongqing. Results The distribution of serum MBL levels in children （28 days to 6 years） was abnormal but there was no significant difference in MBL serum levels in subjects of different ages and genders. The median concentration of MBL in serum was significantly lower in newborns（median： 1597 μg/L, range： 884-1825 μg/L）, cord blood group（ median： 1462 μg/L, range： 0-4604 μg/L）than in other groups （children group median： 2536 μg/L, range 0-7860 μg/L; adult group median： 2920 μg/L, range 98-6495 μg/L ）. While among the other sub-groups aged from 28 days-6 years （28 day group median 2299 μg/L, range 214-4195 μg/L; 6 months-group median 2622 μg/L, range 5-4637 μg/L; 2 years-6 years group 2585 μg/L, range 198-7860 μg/L）, there was no statistically significant difference. The median serum MBL level in normal children aged from 28 days to 6 years was 2563 μg/L and the Pzs-P97.5 was 171-5079 μg/L. Conclusions The distribution of serum MBL levels in children （28 days to 6 years） was abnormal type but there was no statistically significant difference among different age and sex groups. The reference value of P2.5-P97.5 in children （28 days-6 years） was 171- 5079 μg/L.