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人脑胶质瘤性脑水肿与AQP4的关系 被引量：1

Relationship between the glioma edema in human brain and AQP4
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摘要 目的检测人脑胶质细胞瘤组织中的水通道4(AQP4)的表达,探讨水通道在胶质瘤性脑水肿中的表达规律,阐述胶质瘤性脑水肿的发生、发展机制。方法对24例胶质细胞瘤组织进行逆转录-聚合酶链式反应(RT-PCR)和免疫印迹(Western blot),检测胶质细胞瘤组织中AQP4mRNA和AQP4蛋白的表达。结果RT-PCR经半定量分析和Western blot结果表明,胶质细胞瘤组织中AQP4表达高于对照组,二组之间存在显著差异(P<0.01)。结论胶质细胞瘤组织中AQP4mRNA和蛋白质的表达与胶质细胞瘤的恶性程度呈高度正相关,证明了AQP4参与了胶质瘤性脑水肿的发生和发展过程,在胶质瘤性脑水肿的形成过程中起重要作用。 Objective To investigate the expression of aquaporin 4 （AQP4） and its regulation in human brain glioma and to elaborate the mechanism of occurrence and development of glioma edema. Methods The expression of AQP4mRNA and AQP4 protein was detected by reverse transcription polymerase chain reaction （RT-PCR） and Western-Blot analysis in 24 cases of gliomes. Results By semiquantit,ative RT-PCR and Western-Blot analysis, the expression of AQP4mRNA and AQP4 protein in glioma got greater increase than that of the control group. There was significant difference between the two groups （P 〈 0. 01 ）. Conclusion The expression of AQP4mRNA and AQP4 protein in glioma is highly positively related to the malignant grade of glioma. It has been proved that AQP4 takes part in the process of glioma edema and plays an important role in process of glioma edema.
作者 刘伟 只达石
机构地区 天津市环湖医院神经外科
出处 《中华神经外科疾病研究杂志》 CAS 2008年第4期313-316,共4页 Chinese Journal of Neurosurgical Disease Research
关键词 人脑胶质细胞瘤 脑水肿 AQP4 Human glioma Edema Aqupofine4
分类号 R739.41 [医药卫生—肿瘤]
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参考文献10

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二级参考文献23

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共引文献57

同被引文献18

  • 1武弋,章翔,费舟,封亚平,宋少军,王彦刚,贺晓生.犬颅脑火器伤后早期AQP_4的表达及意义[J].中华神经外科疾病研究杂志,2004,3(4):337-340. 被引量：4
  • 2唐忠,袁贤瑞,粟枫,姜维喜,罗瑞午.家兔上矢状窦中1/3及其回流静脉结扎后脑水肿变化和水通道蛋白-4表达的研究[J].中国神经精神疾病杂志,2005,31(6):405-408. 被引量：5
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中华神经外科疾病研究杂志
2008年 第4期

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