应用肿瘤基因芯片筛选早期肺鳞癌相关基因

Detection of associated genes in human lung squamous cell carcinoma in early stage with tumor cDNA microarray

目的:研究人早期肺鳞癌发生相关基因表达谱,探讨肺鳞癌发生的分子机制。方法:选取人早期肺鳞癌组织以及相应正常组织,提取RNA,与含480个与肿瘤相关基因的芯片杂交,结果经SuperArray Image软件分析后比较两种组织中的差异表达基因。结果:共筛查出差异表达基因192条,其中表达上调基因127条,下调基因65条;按照基因功能可分为运输载体、代谢相关基因、细胞信号转导分子、细胞骨架、转录调控因子基因。结论:基因芯片可用于早期肺鳞癌相关基因表达谱的筛查,可为明确早期肺鳞癌发生机制提供重要参考。

AIM： To investigate the gene expression profile of human lung squamous cell carcinoma in early stage. METHODS： The mRNA was extracted from cancer tissue and normal lung tissue. The mixed probes were labeled and hybridized with chip containing 480 carcinoma related genes, then analyzed by SuperArray Image software to study the gene expression patterns in lung squamous cell carcinoma. RESULTS： 192 genes associated with lung squamous cell carcinogenesis were found, which were grouped into transporter, adhesion molecules, cytoskeleton proteins, transcription regulator, genes associated with metabolism and immune response according to functions. 127 gens were positive to lung squa- mous cell carcinogenesis and 65 genes were negative to lung squamous cell carcinogenesis. CONCLUSION ： The screen of gene expression profile of human lung squamous cell carcinoma provides valuable information for the research of molecular mechanism of the lung squamous cell carcinogenesis.