苦参碱对类风湿关节炎外周血淋巴细胞增殖抑制细胞周期影响的研究

The inhibitory effect of Matrine on the proliferation of peripheral lymphocytes of patients with rheumatoid arthritis

目的通过体外实验比较苦参碱（Mat）与甲氨蝶呤（MTX）对类风湿关节炎（RA）患者外周血淋巴细胞（PBL）生长、细胞周期的影响，探讨Mat对RA患者PBL的作用机制。方法应用四甲基偶氮唑蓝（MTF）比色法测定不同时间、不同浓度干预的MTX及Mat吸光度（A）值，计算细胞抑制率；流式细胞术（FCM）检测48hMTX及Mat的细胞周期。结果①MTX、Mat可抑制体外培养的PBL细胞生长，随着药物浓度的增大，A值下降，细胞抑制率（IR％）增加，呈剂量依赖性。48h作用点IR％明显〉24h作用点（P〈0．01）。两药物比较差异无统计学意义（P〉0．05）。②MTX、Mat可使细胞周期构成发生明显变化，与对照组相比差异有统计学意义（P〈0．01），GdG，期细胞比例增高，s期和GJM期细胞比例降低。随着药物浓度增加，细胞增殖指数逐渐降低，呈剂量依赖性。两药物比较差异无统计学意义（P〉0．05）。结论Mat对体外培养的RA患者PBL生长的抑制作用及细胞周期的作用与MTX相当。推测Mat可能具有治疗RA的潜在价值。

Objective To investigate the effects of Matrine （Mat） on the proliferation and cell cycle of the peripheral blood lymphocytes of patients with rheumatoid arthritis. Furthermore, its mechanism of treatment for rheumatoid arthritis （RA） is explored by comparing with methotrexate （MTX）. Methods MTT method was used to measure the light-absorption value of the cells treated with different doses of Mat and MTX at different time, and the inhibition rate（IR% ） was calculated. Flow cytometry was used to measure cell cycle of the cells treated with different doses of Mat and MTX for 48 h. Results （1） Both MTX and Mat could inhibit the proliferation of PBL in patients with RA and the effect was dose-dependent. The IR%was increased （P〈0.05）. IR% at 48 h was higher than IR% at 24 h （P〈0.01）. The two medications had no significant difference （P〉0.05）. （2） Both MTX and Mat could change the cell cycle and acted on GI/S point. They both increased the number of cells in G0/G1 phase and decreased cells in S and GJM phase （P〈0.05）. The two medications had no significant difference （P 〉0.05）. Conclusion Mat inhibits the proliferation and proliferation-dependent processes of PBL in patients with RA. Its effect is comparable to MTX. Mat may have the potential in the trea-tment of RA.