鼠尾悬挂试验在大鼠急性坏死性胰腺炎中的实验意义

Significance of the tail suspension test in rats with acute necrotizing pancreatitis

目的:探讨鼠尾悬挂试验(TST)在大鼠急性坏死性胰腺炎中的实验意义。方法:SD大鼠被分为三组:急性坏死性胰腺炎组(A组,n=8)、急性坏死性胰腺炎鱼油干预组(F组,n=8)和对照组(C组,n=8)。观察各组大鼠胰腺组织病理变化及TST静止时间,同时测定各组5d后血清SOD、GSH-Px活力和淀粉酶浓度。结果:A组、F组TST静止时间各时间段均显著高于C组(P<0.01);自第2天起F组的数值均小于A组,其中第4、第5天的数值差异有显著性(分别为P<0.05、P<0.01)。病理学检查提示F组的炎性细胞浸润显著低于A组(P<0.01)。A组SOD、GSH-Px显著低于C组(P<0.01),而F组SOD、GSH-Px显著高于A组(P<0.01)。TST静止时间和病理学改变、SOD、GSH-Px活力具有相关性(P<0.01)。结论:TST静止时间在一定程度上反映了急性坏死性胰腺炎的严重程度;鱼油在改善急性坏死性胰腺炎大鼠的氧化应激指标的同时,对大鼠的TST静止时间产生影响。

Objective：To investigate the significance of the tail suspension test in rats with acute necrotizing pancreatitis （ANP）. Methods： The male Sprague-Dawley （SD） rats were divided into three groups： ANP group （group A, n=8）, ANP with fish oil treatment group （group F, n=8） and control group （group C, n=8）. The immobility time in the tail suspension test and the grade of the severity of pancreatitis were observed. After experiment started for five days, the activity of serum antioxidant enzymes （SOD, GSH-Px） and the concentration of serum amylase in rats of each group were measured respectively. Results： The immobility time in the tail suspension test of group A and F were significantly longer than that of group C （P〈0.01）. Since the second day after the experiment has started, the immobility time in the tail suspension test of group F was shorter than that of group A. However, only on the 4th and 5th day,this difference showed statistic significance （P〈0.05 and P〈0.01 respectively）. Histopathological analysis suggested the infiltration of that inflammatory cells in group F was significantly weaker than that of group A （P〈0.01）. Serum glutathione peroxidase （GSH-Px） and superoxide dismutase （SOD） level in group A were significantly lower than that in group C （P〈0.01）, and these parameters of group F were significantly higher than those of group A （P〈0.01）. The duration of immobility in the tail suspension test was related to the histopathological changes and the activity of serum SOD and GSH-Px. Conclusion： The duration of immobility in the tail suspension test is correlated with the severity of ANP, and fish oil can affect the ANP rats＇ behaviour, and improves the severity of acute necrotizing Dancreatitis.