孕哺期镧暴露对大鼠子代海马神经元细胞内钙稳态及超微结构影响

Effect of Lanthanum Exposure during Pregnancy and Lactation on Calcium Level in Hippocampus Cell of Baby-rats

[目的]探讨孕哺期镧暴露所致仔代发育期的神经毒性机制。[方法]成年雌性Wister大鼠受孕后至仔鼠出生后21 d,用不同浓度氯化镧(LaCl3)水染毒,仔鼠断乳后处死,取海马检测海马神经元细胞内游离钙离子([Ca2+]i)浓度。[结果]大鼠海马神经元细胞[Ca2+]i浓度(nmol/L),2.5 g/L氯化镧水组为(234.566±19.596),5.0 g/L氯化镧水组为(327.294±34.547),10.0 g/L氯化镧水组为(446.258±29.325),蒸馏水对照组为(159.396±15.235),3个实验组均高于对照组,中剂量组高于低剂量组,高剂量组高于中剂量组,差异均有统计学意义(P<0.01)。显微镜下观察,染毒组海马神经元细胞的超微结构发生病理性改变。[结论]随着氯化镧浓度增加引起相关中枢神经元[Ca2+]i显著增加,并对海马超微结构产生影响。

[Objective]To study the mechanism of neurotoxic effect of lanthanum exposure during pregnancy and lactation on baby-rats. [Methods]The female rats were administrated with lanthanum water from pregnancy to 21th day after delivery,[,Ca^2＋]i level in hippocampus cell of baby-rats was tested. [Results][Ca^2＋ ]i level in hippocampus cell in the rats administrated with 2.5 g/L,10.0 g/L,5.0 g/L of lanthanum water and steamed water in control group was 234. 566±19. 596,327. 294±34. 547,446. 258±29. 325 and 159. 396± 15. 235 respectively. Its near-by cortex in offspring rats exposed to lanthanum showed the increase of[Ca2＋ ]i in middle,medium and high lanthanum-exposed groups compared with the control. [Conclusion]The development of neuron-toxicity induced by lanthanum may be associated with the increase of [Ca^2＋]i in brain. There are significant changes in hippocampug synapses of exposed to lanthanum.