不同来源白头翁药材的皂苷含量比较

Comparison of Content of Pulchinenoside B4 from Different Sources of Pulsatilla Chinensis

目的以白头翁皂苷B4作为指标,考察不同来源白头翁的质量,为白头翁的加工和选用提供参考。方法采用高效液相色谱法,对购自亳州中药材大市场白头翁药材与饮片5个样品和滁州野生白头翁进行含量测定。结果在0.3125～10μg范围内线性关系良好,回归方程Y=276350X-12180,R2=0.9996。结论不同来源的白头翁药材皂苷B4含量差异较大。

Objective To investigate the quality of Pulsatilla chinensis from different sources on reference of pulchinenoside B4 as the index. Methods Five kinds of Pulsatilla chinensis herbs and cut crude drug were purchased from Chinese herbal medicine market in Bozhou, and Wild Pulsatilla chinensis were collected from Chuzhou, The content of pulchinenoside B4 were determined by HPLC. Results Linear relationship was good within 0.312 5-10 μg, the regression equation was Y=276 350X -12 180, R^2= 0.999 6. Conclusion The differences of pulchinenoside B4 from different sources of Pulsatilla chinensis varied greatly.