丹参药材水溶性成分含量的HPLC法分析

Determination of Water Soluble Components in Salvia miltiorrhiza Bge. by HPLC

目的建立丹参药材水溶性成分中丹酚酸B、丹参素和原儿茶醛的含量测定方法。方法采用高效液相色谱法测定,色谱柱为Kromosil C18(4.6mm×250mm,5μm);流动相Ⅰ:甲醇-乙腈-甲酸-水(30∶10∶1∶59),流动相Ⅱ:甲醇-冰醋酸-水(20∶1∶80);流速:1.0mL/min;检测波长分别为286、280nm;柱温35℃。结果测定了10个不同产地的丹参药材,丹酚酸B含量在0.09%～6.81%之间,平均回收率为99.64%,RSD=1.09%;丹参素的含量在0.16%～0.68%之间,平均回收率为98.94%,RSD=1.49%;原儿茶醛的含量在0.01%～0.09%之间,平均回收率为98.96%,RSD=1.66%。结论本法简便、准确、重复性好,可作为丹参药材的含量测定标准。

Objective To establish a method for determination of the water-soluble components of Salvianolic acid B, Danshensu and Protocatechuic aldehyde in Salvia miltiorrhiza Bge. Method Chromatography was performed on Kromosil C18 （4.6 mm×250 mm, 5 μm）. Mobile phase I was methanol-acetonitrile-formicacid-water （30 ： 10 ： 1 ： 59）, mobile phase Ⅱ was methanol-acetic acid-water （20 ： 1 ： 80）, the flow rate was 1.0 mL/min, the detection wavelength was set at 286 and 280 nm, column temperature was 35℃. Result Determined 10 different habitats Salvia miltiorrhiza Bge., the average content of Salvianolic acid B was 0.09%-6.81%, the average recovery was 99.64%, RSD= 1.09%. The average content of Danshensu was 0.16%-0.68%, the average recovery was 98.94%, RSD=1.49%. The average content of Protocatechuic aldehyde was 0.01%-0.09%, the average recovery was 98.96%, RSD= 1.66%. Conclusion The method is accurate and reliable with good reproducibility, and can be used as determination standard for Salvia miltiorrhiza Bge.