自然更年期大鼠IL-2、TNF、TGF和IL-2R mRNA变化及“逆针”关元穴对其影响的研究

Changes of IL-2, TNF, TGF and IL-2R mRNA of Menopausal Rats and Effects of Preventive Acupuncture on Guanyuan Point

目的探讨逆针关元穴对自然更年期大鼠血清、子宫的细胞因子白细胞介素-2(IL-2)、肿瘤坏死因子(TNF)、转化生长因子(TGF)和脾脏白细胞介素-2受体mRNA(IL-2R mRNA)的影响及其机制。方法将112只已经通过阴道涂片筛查的10月龄SD雌性大鼠随机分为7组,另3.5月龄1组16只。采用放射免疫、原位杂交等方法,观察10月龄逆针关元穴(2次/周,连续8周)对随后12、14、16月龄大鼠血清、子宫的细胞因子IL-2、TNF、TGF和脾脏IL-2R mRNA的影响。结果与自然年轻组相比,模型对照各组血清和子宫IL-2、TGF及子宫TNF均下降,血清TNF水平呈先降后升的波动性变化,脾脏IL-2R mRNA表达水平下降;而预先介入逆针疗法后,与自然同月龄组相比,逆针各组血清和子宫IL-2、TGF及子宫TNF上升,其中逆针14、16月龄组血清TGF,逆针12、16月龄组子宫IL-2,逆针12月龄组子宫TNF和逆针12、16月龄组子宫TGF均明显提高(P<0.05,P<0.01,P<0.01,P<0.05,P<0.01,P<0.05,P<0.05)。血清TNF水平与模型对照组呈相反的波动性变化,其中逆针12、14月龄组差异显著(均P<0.05)。脾脏IL-2R mRNA上调,其中逆针14月龄差异显著(P<0.05)。结论逆针关元穴可以调节随后更年期大鼠血清和子宫的细胞因子IL-2、TNF、TGF水平和脾脏IL-2R mRNA的表达,这可能是该疗法改善更年期免疫功能的机制之一。

Objective To observe the effect on IL-2, TNF, TGF in uterus and serum, and IL-2RmRNA in spleen of menopausal rats with preventive accupuncture on Guanyuan point, and analysis the mechanism. 112 ten month-aged SD female rats examined by vaginal smear were chosen and divided into seven groups. And another 16 three point five month-aged SD female rats were another group. Radio-immunity and hybridiztion in situ methods were applied to observe the effect on IL-2, TNF, TGF in uterus and serum and IL-2R mRNA in spleen of 12, 14 and 16 month-aged menopausal rats with preventive accupuncture on Guanyuan point （twice a week, eight weeks continued） in 10th month. Compared with the normal group, IL-2, TGF in uterus and serum and TNF in uterus of menopausal rats reduced, TNF in serum came down in the 12th month and went up in 14th and 16th month, IL-2R mRNA in spleen decreased. Compared with the same-aged model group, IL-2, TGF of serum and uterus and TNF in uterus increased. TGF in serum of 14 and 16 month-aged group, IL-2 in uterus of 12 and 16 month-aged group, TNF in uterus of 12 month-aged group and TGF in uterus of 12 and 16 month-aged group rised significantly （P〈0.05 or P〈0.01）. TNF in serum presented a converse wave-like change and it increased apparently of the 12 and 14 month-aged group （P〈0.05）. IL-2R mRNA increased with preventive acupuncture and it went up distinctly in the 14th month （P〈0.05）. Conclusion Preventive accupuncture on Guanyuan point can improve the immunologic function of menopausal rats, which is probably related to the dominating of IL-2, TNF and TGF in serum and uterus, and IL-2R mRNA in spleen.