金思维对散发性老年性痴呆模型大鼠学习记忆能力的影响

Effect of GETO on Learning and Memory of Sporadic Alzheimer’s Disease Rats

目的观察中药复方金思维对散发性老年性痴呆(SAD)大鼠模型学习记忆的影响。方法采用双侧脑室注射微量链脲佐菌素(STZ)建立散发性AD大鼠模型,金思维分为3个不同剂量对模型大鼠进行3个月的治疗。利用Morris水迷宫检测大鼠空间学习记忆能力并与盐酸多奈哌齐进行比较。结果定位航行实验中,模型组大鼠平均逃避潜伏期和游泳距离较假手术组明显延长(P<0.05或P<0.01);金思维各剂量组和多奈哌齐组平均逃避潜伏期和游泳距离较模型组明显缩短(P<0.05或P<0.01)。空间探索实验中,模型组与假手术组比较,原平台象限活动时间明显缩短(P<0.01);金思维各剂量组与模型组比较,原站台象限活动时间明显延长(P<0.01);与多奈哌齐组相比无显著差异。结论金思维能够显著改善散发性SAD模型大鼠的学习记忆功能。

Objective To establish rat models with Sporadic Alzheimer＇s disease （SAD） induced by intracerebroventricular administration of streptozotocin （ic-STZ）, and observe the effects of compound Jinsiwei （GETO） on leaming and memorizing ability of rats. Methods To establish the Sporadic Alzheimer＇s disease model by intracerebroventricular micro-injection of STZ bilaterally. GETO groups were treated with gastric perfusion of GETO in three different dosage for 3 months. Leaming and memorizing ability of the rats were inspected through Morris water maze test. The effects of GETO and Donepezil were observed and compared. Results In place navigation test, the escape latency and swimming distance were significantly longer in the model group than that in the sham group （P〈0.05 or P〈0.01）, but shorter in GETO groups and Donepezil group （P〈0.05 or P〈0.01）. In spatial probe test, compared with the sham group, swimming time in the platform of previous quadrant was significantly shorter in model group （P 〈0.01）, but significantly longer in GETO groups （P〈0.01）, and there was no difference between Donepezil group and GETO groups. GETO can remarkably improved the learning and memorizing ability of SAD model rats.