丹玄口康对槟榔提取液刺激下的人口腔黏膜成纤维细胞增殖及增殖细胞核抗原表达的影响

Effect of Danxuan Koukang on Proliferation and PCNA Production of Human Oral Mucosal Fibroblasts Induced by Areca Nut Extract

目的观察丹玄口康对槟榔提取液(Areca Nut Extract,ANE)诱导的人口腔黏膜成纤维细胞(Fibroblasts,FB)的增殖及增殖细胞核抗原(Proliferating cell nuclear antigen,PCNA)表达的影响。方法体外培养人口腔黏膜FB,100μg/mL ANE为诱导剂,10%大鼠丹玄口康药物血清为干预物;用二甲基噻唑二苯基四唑溴盐(MTT)法测定细胞增殖速度,用免疫细胞化学法检测PCNA的表达。结果MTT显示,丹玄口康组的OD值低于ANE刺激组(P<0.01或P<0.05),丹玄口康组免疫阳性细胞数及相对灰度值均低于ANE刺激组(P<0.01或P<0.05)。结论丹玄口康能够抑制ANE刺激下的人口腔黏膜FB的增殖,下调PCNA的表达。

Objective To study the effect of Danxuan Koukang （DXKK） drug serum on proliferation and proliferating cell nuclear antigen （PCNA） production of human oral mucosal fibroblasts （FB） induced by areca nut extract （ANE）. Methods Human oral mucosal FB were cultured in vitro, 100 μg/mL ANE was used as inducement and 10% DXKK drug serum as intervenor. The cell proliferation rates were measured by MTT assay, PCNA productions were examined by immunocytechemistry. Results MTT assay showed OD value in DXKK groups were significantly decreased compared with the induced group （P〈0.01 or P〈0.05）. Immunocytechemistry showed counts and relative grey value of PCNA-positive cells in DXKK groups were significantly decreased compared with the induced group （P〈0.01 or P〈0.05）. Conclusion DXKK can inhibit proliferation and PCNA production of human oral mucosal FB induced by ANE.