通塞脉微丸和丁基苯酞对PC12细胞缺氧缺糖损伤模型的保护作用

Protective Effect of Tongsaimai Pellets and Butylphthalide to PC12 Cell in Hypoxia/Hypoglycemia Model

目的探讨通塞脉微丸和丁基苯酞对PC12细胞缺氧缺糖损伤模型的保护作用。方法以肾上腺嗜铬瘤克隆化细胞株PC12细胞为材料,制备缺氧、缺糖损伤模型;MTT法测定PCl2细胞存活数及乳酸脱清酶法测定细胞损伤程度。结果形态学检查发现,通塞脉微丸和丁基苯酞对两种损伤模型中的PC12细胞具有明显保护作用,MTT染色和乳酸脱氢酶活性测定显示,通塞脉微丸和丁基苯酞可显著提高损伤模型中PC12细胞的存活数,降低细胞损伤程度,其中以对缺氧损伤保护作用尤为显著。结论通塞脉微丸和丁基苯酞对PC12细胞缺氧和缺糖损伤模型均有显著保护作用。

Objective To discuss the protective effect of Tongsaimai pellets （TSMP） and butylphthalide （NBP） on PC12 cell in the hypoxia/hypoglycemia model. Methods Two ischemia models including hypoxia, hypoglycemia models were used to assay the anti-ischemic roles of TSMP and NBP in cultured PC12. TSMP and NBP possessed obvious protective effects on PC12 cells from two injured models. Both of them could increase the number of living cells and decrease LDH activity significantly, particularly in hypoglycemia injured model. Conclusion TSM and NBP have protective effect on PC 12 cells from two injured models effectively in vitro.