加味四逆颗粒大孔树脂精制工艺研究

Study on Pureparation Techniques of Jiawei Sini Granules

目的对加味四逆颗粒大孔树脂吸附精制的方法进行工艺考察,选取最佳工艺条件。方法采用正交试验设计,以柴胡皂苷A、芍药苷、橙皮苷、甘草酸4种成分为指标,采用HPLC法对上述指标成分进行含量测定。结果选用AB-8型大孔树脂,每克树脂的最大吸附量分别为柴胡皂苷A 2.8 mg、芍药苷62.8 mg、橙皮苷49.6 mg、甘草酸84.9 mg,上样药液浓度为0.5 g(原药材)/mL、树脂柱的径高比为1∶7、吸附流速为2 BV/h为最佳上样工艺。结论大孔树脂吸附法可用于加味四逆颗粒的精制。

Objective To optimize the conditions of Jiawei Sini granules by inspecting the macroporous resin adsorption refined methods. Methods Orthogonal design was used with saikosaponin A, paeoniflorin, hesperidin and glycyrrhizin as indicators determined by HPLC. Results The AB-8 macroporous resin, the biggest per gram of resin adsorption capacity were saikosaponin A 2.8 mg, paeoniflorin 62.8 mg, hesperidin 49.6 mg, glycyrrhizin 84.9 mg, with the concentration of durg 0.5 g/mL, the resin column diameter-height ratio of 1 ： 7 and adsorption velocity of 2 （BV/h）. Conclusion Macroporous resin adsorption method can be used for refining Jiawei Sini Granules.