反相高效液相色谱法测定化妆品中的3-O-乙基维生素C

Determination of 3-O-ethyl-ascorbic acid by reversed phase high performance liquid chromatography

采用反相高效液相色谱法测定化妆品中的3—O-乙基维生素C，色谱条件：SHIMADZU Shim-pack VP-C18ODS（150mm×4．6mm，5μm）色谱柱；C18ODS保护柱芯；流动相V（甲醇）：V（0．025mol/L KH2PO4）=20：80；流速：1．0mL/min；柱温：30℃；紫外检测器检测，检测池温度：40℃，波长：254nm。结果表明，在此条件下，3—O-乙基维生素C在0-100mg／L与相应的峰面积具有良好的线性关系（r=0．9992），线性回归方程为A=31．1703ρ，回收率在97．75％-100．60％，方法精密度RSD约为2．6％（n=5）。

A reversed phase high performance liquid chromatography （RP -HPLC ） method for the determination of 3 - O - ethyl - ascorbie acid in cosmetics was established. The sample was analyzed on a SHIMADZU Shim -pack VP -C18ODS （150 mm ×4. 6 mm, 5 μm） with C18ODS guard column core and UV detector. Operating conditions are： mobile phase consisted of V（ CH3OH）： V（ KH2PO4 solution with concention 0. 025 mol/L）=20：80 at a flow rate of 1.0 mL/min; column temperature 30 ℃ ; wavelength of the UV detector 254 nm ; detection cell temperature 40 ℃. Results showed that as the concentration of 3 - O - ethyl - ascorbic acid is in the range of 0 - 100 mg/L, the relationship between concentration and the corresponding peak area shows good linearity （r = 0. 999 2）, The regression equation can be expressed as A = 31. 170 3ρ and the recovery achieves 97. 75% - 100. 60%. The RSD of the method is 2. 6% （n =5）,