樗白皮提取物对溃疡性小鼠脾脏与血清中腺苷脱氨酶的影响

Effects of extracts from Ailanthus altissima on the contents of adenosine deaminase in spleen and serum of mice infected with ulcerous colitis

为了探讨樗白皮不同活性成分对溃疡性结肠炎防治作用,以小白鼠为实验动物,随机分为正常对照组、模型组、水提取物组、水提醇沉提取物组、95%乙醇提取物组、65%乙醇提取物组、45%乙醇提取物组、25%乙醇提取物组。以2,4-二硝基氯苯制备小鼠溃疡性结肠炎模型,以各种提取物灌胃模型小鼠,7d后,测定血清及脾脏组织中腺苷脱氨酶(ADA)活性。结果,与模型组相比,水提醇沉物能显著地降低血清和脾脏(P<0.01)中ADA的含量,45%醇提物能降低血清与脾脏匀浆(P<0.01)ADA的含量,25%醇提物也能降低血清和脾匀浆(P<0.05)中ADA的含量,65%醇提物组仅对脾脏匀浆中ADA含量降低有作用(P<0.05),而对血清中ADA含量的降低无差异显著性,95%醇提物和水提物对血清与脾匀浆ADA含量无显著性影响。表明樗白皮中含有降低溃疡性结肠炎时腺苷脱氨酶的活性成分,能影响组织细胞核酸代谢,对溃疡性结肠炎具有一定的防治作用。

Objective： To investigate the treatment function of different extracts from Ailanthus altissima on mice infected with ulcerous colitis. Mothods： 80 Kunming mice were randomly divided into 8 groups： control, model, extract of water, extract of water- alcohol deposit, extract of 95% alcohol, extract of 65% alcohol, extract of 45% alcohol, extract of 25% alcohol. Mice model of ulcerous colitis was made by 2,4 -dinitroehlorn- benzene （DNCB） , each extract was given to the mouse by intragastrie administration. After 7 days, the contents of adenosine deaminase （ADA） in serum and spleen were determined. Results： Compared with the model group, the contents of ADA in serum and spleen decreased in water-alcohol deposit group （P 〈 0.01 ）, as while as 45% alcohol group （P〈0.01） and 25% alcohol group （P〈0. 01 or P〈0.05）. The extract of 653 alcohol could decreased the contents of ADA in spleen（ P 〈0.05）. While the extract in 95% alcohol and water groups had no influence on the activity of ADA. Conclusion： The effective constituent in Ailanthus altissima could decrease the activity of ADA, adjust the metabolism of nucleic acid and treat ulcerous colitis.