A2N双污泥反硝化除磷系统微生物的先间歇后连续培养及快速启动

First Sequencing Then Connecting Cultivation of the Organisms and Fast Start-up of A_2N Two-Sludge System

以实际生活污水为对象,研究了反硝化聚磷菌(DPB)的驯化培养以及A2N双污泥反硝化除磷系统的快速启动.采用先独立培养反硝化聚磷菌和好氧硝化生物膜再连续运行的方式成功地快速启动了A2N系统.采用污水处理厂除磷工艺中的活性污泥为种泥,在SBR系统中以先A/O(厌氧/好氧)后A/A(厌氧/缺氧)的方式运行,32 d成功地使反硝化聚磷菌成为优势菌属.在SBR反应器中,采用硝化效果较好的活性污泥为种泥,好氧硝化生物膜30 d挂膜成功,氨氮去除率稳定在99%以上.然后,A2N系统连续运行,11 d后系统反硝化除磷效果进入稳定状态,出水氨氮和正磷酸盐浓度均为0,硝态氮为10.26 mg/L,出水COD为19.56 mg/L,COD、氨氮、总氮和磷去除率分别为91%、100%、77%和100%,说明A2N系统具有很好的脱氮除磷效果,认为系统启动成功.

Using domestic sewage,the cultivation of denitrifying phosphorus removing bacteria（DPB） and start-up of A2N two-sludge system were investigated in a laboratory.The A2N system started up fast by using the method that first bringing up DPB and nitrification biofilm separately then connecting them in the A2N system.The enrichment of DPB used activated sludge from the sedimentation tank of a wastewater treatment plant that working about phosphorus removal.In a sequencing batch reactor（SBR）,the sludge mixture was operated under anaerobic-aerobic（A/O） condition firstly.Then,the activated sludge was conducted under anaerobic-anoxic（A/A） condition.During the experiment period of 32 d,DPB was successfully enriched.At the same time,utilizing the activated sludge that enriched of nitrifying bacteria,nitrification biofilm was grown up in another SBR.After 30 d,the average ammonia removal efficiency steadily stayed above 99%.Later,using the nitrification biofilm and the activated sludge that enriched of DPB,the A2N system was connected for dealing with domestic sewage.After 11 d,the treatment effect of A2N system attained steady.The total nitrogen and phosphorus concentration of effluent are 0,the nitrate concentration is 10.26mg/L, and COD is 19.56mg/L.The removal efficiency of COD,ammonia,total nitrogen and phosphorus are 91%,100%,77% and 100%,respectively.Each result shows that the A2N system is started up successfully.