兔胆囊上皮细胞的一种体外原代培养方法

A Method of Primary culture of Rabbit Gallbladder Epithelial cells in vitro

以RPMI－1640为基础培养液，添加适量小牛血清、表皮生长因子等营养成分，在37℃、5％CO2的湿润空气培养箱内培养兔胆囊上皮细胞，细胞经10天左右形成融合单层，细胞形态呈卵圆状，单层可维持十天以上。

Gallbladder epithelial cells from rabbit were cultured in RPMI-1640 medium with 10% ~ 15% fetal calf serum, 10ng/ml EGF at 37℃ humid 5% CO2. In about 10 days the cells reached a confluent,monolayer these cells appeared as oblate spheroid. The monolayer could maintained more than 10 days