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丝羽乌骨鸡β-防御素基因的克隆和序列分析 被引量:2

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摘要 用设计的特异性引物,采用RT-PCR技术特异性地扩增到丝羽乌骨鸡β-防御素基因Gal-1(Gallinacin-1),Gal-1(a)(Gallinacin-1(a)),Gal-2(Gallinacin-2),Gal-4(Gallinacin-4)~Gal-13(Gallinacin-13)基因共13个基因编码区全长片段。通过克隆、测序获得13个基因的cDNA核苷酸序列,并提交到GenBank,丝羽乌骨鸡β-防御素Gal-1,Gal-1(a),Gal-2,Gal-4~Gal-13基因的登录号为:DQ677632 ̄DQ677644。将丝羽乌骨鸡13种β-防御素Gal-1~Gal-13基因分别与GenBank中收录的防御素基因比对分析,其氨基酸序列的相似性均在95.5%~100%之间。利用DNAStar软件对所获得的13种丝羽乌骨鸡β-防御素基因进行系统发育树分析,结果Gal-1、5、12在同一分支上,Gal-1(a)、4、8在同一分支上,Gal-1、2、9在同一分支,Gal-6、7在同一分支,Gal-13独在一分支上。
出处 《广东畜牧兽医科技》 2008年第4期22-24,44,共4页 Guangdong Journal of Animal and Veterinary Science
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参考文献12

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